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Structure paper

TitleCatalytic Determinants of Alkene Production by the Cytochrome P450 Peroxygenase OleTJE.
Journal, issue, pagesJ. Biol. Chem., Vol. 292, Page 5128-5143, Year 2017
Publish dateOct 5, 2016 (structure data deposition date)
AuthorsMatthews, S. / Belcher, J.D. / Tee, K.L. / Girvan, H.M. / McLean, K.J. / Rigby, S.E. / Levy, C.W. / Leys, D. / Parker, D.A. / Blankley, R.T. / Munro, A.W.
External linksJ. Biol. Chem. / PubMed:28053093
MethodsX-ray diffraction
Resolution1.44 - 1.95 Å
Structure data

PDB-5m0n:
Crystal structure of cytochrome P450 OleT in complex with formate
Method: X-RAY DIFFRACTION / Resolution: 1.44 Å

PDB-5m0o:
Crystal structure of cytochrome P450 OleT H85Q in complex with arachidonic acid
Method: X-RAY DIFFRACTION / Resolution: 1.8 Å

PDB-5m0p:
Crystal structure of cytochrome P450 OleT F79A in complex with arachidonic acid
Method: X-RAY DIFFRACTION / Resolution: 1.95 Å

Chemicals

ChemComp-HEM:
PROTOPORPHYRIN IX CONTAINING FE

ChemComp-SO4:
SULFATE ION

ChemComp-FMT:
FORMIC ACID

ChemComp-HOH:
WATER

ChemComp-EPA:
5,8,11,14,17-EICOSAPENTAENOIC ACID

ChemComp-DCR:
icosanoic acid

ChemComp-NA:
Unknown entry

Source
  • jeotgalicoccus sp. atcc 8456 (bacteria)
KeywordsOXIDOREDUCTASE / cytochrome P450 / decarboxylase / oxidase / peroxide

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