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TitleMembrane-assisted viral DNA ejection.
Journal, issue, pagesBiochim Biophys Acta Gen Subj, Vol. 1861, Issue 3, Page 664-672, Year 2017
Publish dateDec 16, 2016
AuthorsIsaac Santos-Pérez / Hanna M Oksanen / Dennis H Bamford / Felix M Goñi / David Reguera / Nicola G A Abrescia /
PubMed AbstractGenome packaging and delivery are fundamental steps in the replication cycle of all viruses. Icosahedral viruses with linear double-stranded DNA (dsDNA) usually package their genome into a preformed, ...Genome packaging and delivery are fundamental steps in the replication cycle of all viruses. Icosahedral viruses with linear double-stranded DNA (dsDNA) usually package their genome into a preformed, rigid procapsid using the power generated by a virus-encoded packaging ATPase. The pressure and stored energy due to this confinement of DNA at a high density is assumed to drive the initial stages of genome ejection. Membrane-containing icosahedral viruses, such as bacteriophage PRD1, present an additional architectural complexity by enclosing their genome within an internal membrane vesicle. Upon adsorption to a host cell, the PRD1 membrane remodels into a proteo-lipidic tube that provides a conduit for passage of the ejected linear dsDNA through the cell envelope. Based on volume analyses of PRD1 membrane vesicles captured by cryo-electron tomography and modeling of the elastic properties of the vesicle, we propose that the internal membrane makes a crucial and active contribution during infection by maintaining the driving force for DNA ejection and countering the internal turgor pressure of the host. These novel functions extend the role of the PRD1 viral membrane beyond tube formation or the mere physical confinement of the genome. The presence and assistance of an internal membrane might constitute a biological advantage that extends also to other viruses that package their linear dsDNA to high density within an internal vesicle.
External linksBiochim Biophys Acta Gen Subj / PubMed:27993658
MethodsEM (tomography)
Structure data

EMDB-3548:
Putative initial stage of viral DNA ejection in membrane-containing bacteriophage PRD1
Method: EM (tomography)

EMDB-3549:
Putative intermediate stage of viral DNA ejection in membrane-containing bacteriophage PRD1
Method: EM (tomography)

EMDB-3550:
Putative final stage of viral DNA ejection in membrane-containing bacteriophage PRD1
Method: EM (tomography)

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