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TitleStructure of a 30S pre-initiation complex stalled by GE81112 reveals structural parallels in bacterial and eukaryotic protein synthesis initiation pathways.
Journal, issue, pagesNucleic Acids Res, Vol. 45, Issue 4, Page 2179-2187, Year 2017
Publish dateFeb 28, 2017
AuthorsJorge P López-Alonso / Attilio Fabbretti / Tatsuya Kaminishi / Idoia Iturrioz / Letizia Brandi / David Gil-Carton / Claudio O Gualerzi / Paola Fucini / Sean R Connell /
PubMed AbstractIn bacteria, the start site and the reading frame of the messenger RNA are selected by the small ribosomal subunit (30S) when the start codon, typically an AUG, is decoded in the P-site by the ...In bacteria, the start site and the reading frame of the messenger RNA are selected by the small ribosomal subunit (30S) when the start codon, typically an AUG, is decoded in the P-site by the initiator tRNA in a process guided and controlled by three initiation factors. This process can be efficiently inhibited by GE81112, a natural tetrapeptide antibiotic that is highly specific toward bacteria. Here GE81112 was used to stabilize the 30S pre-initiation complex and obtain its structure by cryo-electron microscopy. The results obtained reveal the occurrence of changes in both the ribosome conformation and initiator tRNA position that may play a critical role in controlling translational fidelity. Furthermore, the structure highlights similarities with the early steps of initiation in eukaryotes suggesting that shared structural features guide initiation in all kingdoms of life.
External linksNucleic Acids Res / PubMed:27986852 / PubMed Central
MethodsEM (single particle)
Resolution13.5 Å
Structure data

3494

5me0

EMDB-3494, PDB-5me0:
Structure of the 30S Pre-Initiation Complex 1 (30S IC-1) Stalled by GE81112
Method: EM (single particle) / Resolution: 13.5 Å

3495

5me1

EMDB-3495, PDB-5me1:
Structure of the 30S Pre-Initiation Complex 2 (30S IC-2) Stalled by GE81112
Method: EM (single particle) / Resolution: 13.5 Å

Chemicals

ChemComp-FME:
N-FORMYLMETHIONINE / N-Formylmethionine

Source
  • Escherichia coli (E. coli)
  • escherichia coli k-12 (bacteria)
  • thermus thermophilus (strain hb8 / atcc 27634 / dsm 579) (bacteria)
  • geobacillus stearothermophilus (bacteria)
KeywordsRIBOSOME / Initiation of Translation

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