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TitleThe N14 anti-afamin antibody Fab: a rare VL1 CDR glycosylation, crystallographic re-sequencing, molecular plasticity and conservative versus enthusiastic modelling.
Journal, issue, pagesActa Crystallogr D Struct Biol, Vol. 72, Page 1267-1280, Year 2016
Publish dateJun 4, 2016 (structure data deposition date)
AuthorsNaschberger, A. / Furnrohr, B.G. / Lenac Rovis, T. / Malic, S. / Scheffzek, K. / Dieplinger, H. / Rupp, B.
External linksActa Crystallogr D Struct Biol / PubMed:27917827
MethodsX-ray diffraction
Resolution1.86 - 1.88 Å
Structure data

PDB-5l7x:
Afamin antibody fragment, N14 Fab, L1- glycosylated, crystal form II
Method: X-RAY DIFFRACTION / Resolution: 1.86 Å

PDB-5l88:
AFAMIN ANTIBODY FRAGMENT, N14 FAB, L1- GLYCOSILATED, CRYSTAL FORM I, non-parsimonious model
Method: X-RAY DIFFRACTION / Resolution: 1.88 Å

PDB-5l9d:
AFAMIN ANTIBODY FRAGMENT, N14 FAB, L1- GLYCOSYLATED, CRYSTAL FORM I, parsimonious model
Method: X-RAY DIFFRACTION / Resolution: 1.88 Å

PDB-5lgh:
Afamin antibody fragment, N14 Fab, L1- glycosilated, crystal form II, same as 5L7X, but isomorphous setting indexed same as 5L88, 5L9D
Method: X-RAY DIFFRACTION / Resolution: 1.86 Å

Chemicals

ChemComp-PG4:
TETRAETHYLENE GLYCOL / precipitant*YM

ChemComp-PEG:
DI(HYDROXYETHYL)ETHER

ChemComp-1PE:
PENTAETHYLENE GLYCOL / precipitant*YM

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose

ChemComp-HOH:
WATER

ChemComp-PE8:
3,6,9,12,15,18,21-HEPTAOXATRICOSANE-1,23-DIOL

ChemComp-PGE:
TRIETHYLENE GLYCOL

ChemComp-K:
Unknown entry

Source
  • mus musculus (house mouse)
KeywordsIMMUNE SYSTEM / Fab / Antibody Fragment / glycosilated / Afamin / Afamin antibody / Fab Fragment / IgG1-kappa / light chain glycosilation

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