EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Modular Assembly of the Bacterial Large Ribosomal Subunit.
ジャーナル・号・ページ	Cell, Vol. 167, Issue 6, Page 1610-1622.e15, Year 2016
掲載日	2016年12月1日
著者	Joseph H Davis / Yong Zi Tan / Bridget Carragher / Clinton S Potter / Dmitry Lyumkis / James R Williamson /
PubMed 要旨	The ribosome is a complex macromolecular machine and serves as an ideal system for understanding biological macromolecular assembly. Direct observation of ribosome assembly in vivo is difficult, as ...The ribosome is a complex macromolecular machine and serves as an ideal system for understanding biological macromolecular assembly. Direct observation of ribosome assembly in vivo is difficult, as few intermediates have been isolated and thoroughly characterized. Herein, we deploy a genetic system to starve cells of an essential ribosomal protein, which results in the accumulation of assembly intermediates that are competent for maturation. Quantitative mass spectrometry and single-particle cryo-electron microscopy reveal 13 distinct intermediates, which were each resolved to ∼4-5 Å resolution and could be placed in an assembly pathway. We find that ribosome biogenesis is a parallel process, that blocks of structured rRNA and proteins assemble cooperatively, and that the entire process is dynamic and can be "re-routed" through different pathways as needed. This work reveals the complex landscape of ribosome assembly in vivo and provides the requisite tools to characterize additional assembly pathways for ribosomes and other macromolecular machines.
リンク	Cell / PubMed:27912064 / PubMed Central
手法	EM (単粒子)
解像度	3.7 - 7.9 Å
構造データ	EMDB-8434: Structure of bL17-depleted large ribosomal subunit assembly intermediate - Class A 手法: EM (単粒子) / 解像度: 6.5 Å EMDB-8440: Structure of bL17-depleted large ribosomal subunit assembly intermediate - Class B 手法: EM (単粒子) / 解像度: 4.5 Å EMDB-8441: Structure of bL17-depleted large ribosomal subunit assembly intermediate - Class C 手法: EM (単粒子) / 解像度: 3.7 Å EMDB-8442: Structure of bL17-depleted large ribosomal subunit assembly intermediate - Class C1 手法: EM (単粒子) / 解像度: 4.5 Å EMDB-8443: Structure of bL17-depleted large ribosomal subunit assembly intermediate - Class C2 手法: EM (単粒子) / 解像度: 4.6 Å EMDB-8444: Structure of bL17-depleted large ribosomal subunit assembly intermediate - Class C3 手法: EM (単粒子) / 解像度: 4.0 Å EMDB-8445: Structure of bL17-depleted large ribosomal subunit assembly intermediate - Class D 手法: EM (単粒子) / 解像度: 4.0 Å EMDB-8446: Structure of bL17-depleted large ribosomal subunit assembly intermediate - Class D1 手法: EM (単粒子) / 解像度: 4.7 Å EMDB-8447: Structure of bL17-depleted large ribosomal subunit assembly intermediate - Class D2 手法: EM (単粒子) / 解像度: 4.9 Å EMDB-8448: Structure of bL17-depleted large ribosomal subunit assembly intermediate - Class D3 手法: EM (単粒子) / 解像度: 4.6 Å EMDB-8449: Structure of bL17-depleted large ribosomal subunit assembly intermediate - Class D4 手法: EM (単粒子) / 解像度: 4.7 Å EMDB-8450: Structure of bL17-depleted large ribosomal subunit assembly intermediate - Class E 手法: EM (単粒子) / 解像度: 3.7 Å EMDB-8451: Structure of bL17-depleted large ribosomal subunit assembly intermediate - Class E1 手法: EM (単粒子) / 解像度: 4.2 Å EMDB-8452: Structure of bL17-depleted large ribosomal subunit assembly intermediate - Class E2 手法: EM (単粒子) / 解像度: 4.5 Å EMDB-8453: Structure of bL17-depleted large ribosomal subunit assembly intermediate - Class E3 手法: EM (単粒子) / 解像度: 5.0 Å EMDB-8455: Structure of bL17-depleted large ribosomal subunit assembly intermediate - Class E4 手法: EM (単粒子) / 解像度: 4.5 Å EMDB-8456: Structure of bL17-depleted large ribosomal subunit assembly intermediate - Class E5 手法: EM (単粒子) / 解像度: 4.9 Å EMDB-8457: Structure of 30S Ribosome - Class F 手法: EM (単粒子) / 解像度: 7.9 Å
由来	Escherichia coli (大腸菌)