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TitleStructural analysis reveals the flexible C-terminus of Nop15 undergoes rearrangement to recognize a pre-ribosomal RNA folding intermediate.
Journal, issue, pagesNucleic Acids Res, Vol. 45, Issue 5, Page 2829-2837, Year 2017
Publish dateMar 17, 2017
AuthorsJun Zhang / Lauren E Gonzalez / Traci M Tanaka Hall /
PubMed AbstractThe RNA recognition motif (RRM) is the most abundant RNA-binding domain in eukaryotes, and it plays versatile roles in RNA metabolism. Despite its abundance, diversity of RRM structure and function ...The RNA recognition motif (RRM) is the most abundant RNA-binding domain in eukaryotes, and it plays versatile roles in RNA metabolism. Despite its abundance, diversity of RRM structure and function is generated by variations on a conserved core. Yeast Nop15 is an RRM protein that is essential for large ribosomal subunit biogenesis. We determined a 2.0 Å crystal structure of Nop15 that reveals a C-terminal α-helical region obscures its canonical RNA-binding surface. Small-angle X-ray scattering, NMR and RNA-binding analyses further reveal that the C-terminal residues of Nop15 are highly flexible, but essential for tight RNA binding. Moreover, comparison with a recently reported cryo-electron microscopy structure indicates that dramatic rearrangement of the C-terminal region of Nop15 in the pre-ribosome exposes the RNA-binding surface to recognize the base of its stem-loop target RNA and extends a newly-formed α helix to the distal loop where it forms protein interactions.
External linksNucleic Acids Res / PubMed:27789691 / PubMed Central
MethodsSAS (X-ray synchrotron) / X-ray diffraction
Resolution2 Å
Structure data

SASDBG6:
Ribosome biogenesis protein 15 (Nop15) (Ribosome biogenesis protein 15, Nop15)
Method: SAXS/SANS

PDB-5t9p:
Structural analysis reveals the flexible C-terminus of Nop15 undergoes rearrangement to recognize a pre-ribosomal RNA folding intermediate
Method: X-RAY DIFFRACTION / Resolution: 2.0 Å

Chemicals

ChemComp-SO4:
SULFATE ION

ChemComp-CL:
Unknown entry

ChemComp-HOH:
WATER

Source
  • Saccharomyces cerevisiae (brewer's yeast)
  • saccharomyces cerevisiae (strain atcc 204508 / s288c) (yeast)
KeywordsRIBOSOMAL PROTEIN / Nop15 / RRM

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