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TitleCryo-EM structure of the large subunit of the spinach chloroplast ribosome.
Journal, issue, pagesSci Rep, Vol. 6, Page 35793, Year 2016
Publish dateOct 20, 2016
AuthorsTofayel Ahmed / Zhan Yin / Shashi Bhushan /
PubMed AbstractProtein synthesis in the chloroplast is mediated by the chloroplast ribosome (chloro-ribosome). Overall architecture of the chloro-ribosome is considerably similar to the Escherichia coli (E. coli) ...Protein synthesis in the chloroplast is mediated by the chloroplast ribosome (chloro-ribosome). Overall architecture of the chloro-ribosome is considerably similar to the Escherichia coli (E. coli) ribosome but certain differences are evident. The chloro-ribosome proteins are generally larger because of the presence of chloroplast-specific extensions in their N- and C-termini. The chloro-ribosome harbours six plastid-specific ribosomal proteins (PSRPs); four in the small subunit and two in the large subunit. Deletions and insertions occur throughout the rRNA sequence of the chloro-ribosome (except for the conserved peptidyl transferase center region) but the overall length of the rRNAs do not change significantly, compared to the E. coli. Although, recent advancements in cryo-electron microscopy (cryo-EM) have provided detailed high-resolution structures of ribosomes from many different sources, a high-resolution structure of the chloro-ribosome is still lacking. Here, we present a cryo-EM structure of the large subunit of the chloro-ribosome from spinach (Spinacia oleracea) at an average resolution of 3.5 Å. High-resolution map enabled us to localize and model chloro-ribosome proteins, chloroplast-specific protein extensions, two PSRPs (PSRP5 and 6) and three rRNA molecules present in the chloro-ribosome. Although comparable to E. coli, the polypeptide tunnel and the tunnel exit site show chloroplast-specific features.
External linksSci Rep / PubMed:27762343 / PubMed Central
MethodsEM (single particle)
Resolution3.5 Å
Structure data

9572

5h1s

EMDB-9572, PDB-5h1s:
Structure of the large subunit of the chloro-ribosome
Method: EM (single particle) / Resolution: 3.5 Å

Source
  • spinacia oleracea (spinach)
KeywordsRIBOSOME / Cryo-EM / chloro-ribosome

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