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TitleThe NuA4 Core Complex Acetylates Nucleosomal Histone H4 through a Double Recognition Mechanism.
Journal, issue, pagesMol Cell, Vol. 63, Issue 6, Page 965-975, Year 2016
Publish dateSep 15, 2016
AuthorsPeng Xu / Chengmin Li / Zhihong Chen / Shuanying Jiang / Shilong Fan / Jiawei Wang / Junbiao Dai / Ping Zhu / Zhucheng Chen /
PubMed AbstractNuA4 catalyzes the acetylation of nucleosomes at histone H4, which is a well-established epigenetic event, controlling many genomic processes in Saccharomyces cerevisiae. Here we report the crystal ...NuA4 catalyzes the acetylation of nucleosomes at histone H4, which is a well-established epigenetic event, controlling many genomic processes in Saccharomyces cerevisiae. Here we report the crystal structures of the NuA4 core complex and a cryoelectron microscopy structure with the nucleosome. The structures show that the histone-binding pocket of the enzyme is rearranged, suggesting its activation. The enzyme binds the histone tail mainly through the target lysine residue, with a preference for a small residue at the -1 position. The complex engages the nucleosome at the dish face and orients its catalytic pocket close to the H4 tail to achieve selective acetylation. The combined data reveal a space-sequence double recognition mechanism of the histone tails by a modifying enzyme in the context of the nucleosome.
External linksMol Cell / PubMed:27594449
MethodsEM (single particle) / X-ray diffraction
Resolution2.7 - 7.9 Å
Structure data

EMDB-9536:
Structure of NuA4 core complex binds with the nucleosome
Method: EM (single particle) / Resolution: 7.9 Å

PDB-5j9q:
Crystal structure of the NuA4 core complex
Method: X-RAY DIFFRACTION / Resolution: 3.25 Å

PDB-5j9t:
Crystal structure of the NuA4 core complex
Method: X-RAY DIFFRACTION / Resolution: 2.7 Å

PDB-5j9u:
Crystal structure of the NuA4 core complex
Method: X-RAY DIFFRACTION / Resolution: 2.95 Å

PDB-5j9w:
Crystal structure of the NuA4 core complex
Method: X-RAY DIFFRACTION / Resolution: 2.8 Å

Chemicals

ChemComp-HOH:
WATER

ChemComp-ACO:
ACETYL COENZYME *A

Source
  • Pichia norvegensis (yeast)
  • saccharomyces cerevisiae (strain atcc 204508 / s288c) (yeast)
  • saccharomyces cerevisiae (brewer's yeast)
KeywordsTRANSFERASE / NuA4 / nucleosome / histone / acetylation

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