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Structure paper

TitleAn Evolved Orthogonal Enzyme/Cofactor Pair.
Journal, issue, pagesJ. Am. Chem. Soc., Vol. 138, Page 12451-12458, Year 2016
Publish dateMay 5, 2016 (structure data deposition date)
AuthorsReynolds, E.W. / McHenry, M.W. / Cannac, F. / Gober, J.G. / Snow, C.D. / Brustad, E.M.
External linksJ. Am. Chem. Soc. / PubMed:27575374
MethodsX-ray diffraction
Resolution2.162 - 2.34 Å
Structure data

PDB-5jqu:
Crystal structure of Cytochrome P450 BM3 heme domain G265F/T269V/L272W/L322I/F405M/A406S (WIVS-FM) variant with iron(III) deuteroporphyrin IX bound
Method: X-RAY DIFFRACTION / Resolution: 2.162 Å

PDB-5jqv:
Crystal structure of Cytochrome P450 BM3 heme domain T269V/L272W/L322I/A406S (WIVS) variant with iron(III) deuteroporphyrin IX bound
Method: X-RAY DIFFRACTION / Resolution: 2.34 Å

Chemicals

ChemComp-FDE:
FE(III) DEUTEROPORPHYRIN IX

ChemComp-HOH:
WATER

Source
  • bacillus megaterium (strain atcc 14581 / dsm 32 / jcm 2506 / nbrc 15308 / ncimb 9376 / nctc 10342 / vkm b-512) (bacteria)
KeywordsOXIDOREDUCTASE / P450 BM3 / iron(III) deuteroporphyrin IX

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