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TitleCrystal structure of SgcJ, an NTF2-like superfamily protein involved in biosynthesis of the nine-membered enediyne antitumor antibiotic C-1027.
Journal, issue, pagesJ Antibiot (Tokyo), Vol. 69, Page 731-740, Year 2016
Publish dateNov 27, 2012 (structure data deposition date)
AuthorsHuang, T. / Chang, C.Y. / Lohman, J.R. / Rudolf, J.D. / Kim, Y. / Chang, C. / Yang, D. / Ma, M. / Yan, X. / Crnovcic, I. ...Huang, T. / Chang, C.Y. / Lohman, J.R. / Rudolf, J.D. / Kim, Y. / Chang, C. / Yang, D. / Ma, M. / Yan, X. / Crnovcic, I. / Bigelow, L. / Clancy, S. / Bingman, C.A. / Yennamalli, R.M. / Babnigg, G. / Joachimiak, A. / Phillips, G.N. / Shen, B.
External linksJ Antibiot (Tokyo) / PubMed:27406907
MethodsX-ray diffraction
Resolution1.7 - 2.719 Å
Structure data

PDB-4i4k:
Streptomyces globisporus C-1027 9-membered enediyne conserved protein SgcE6
Method: X-RAY DIFFRACTION / Resolution: 1.7 Å

PDB-4ovm:
Crystal structure of SgcJ protein from Streptomyces carzinostaticus
Method: X-RAY DIFFRACTION / Resolution: 2.719 Å

Chemicals

ChemComp-CIT:
CITRIC ACID

ChemComp-PG4:
TETRAETHYLENE GLYCOL / precipitant*YM

ChemComp-GOL:
GLYCEROL

ChemComp-PO4:
PHOSPHATE ION

ChemComp-1PE:
PENTAETHYLENE GLYCOL / precipitant*YM

ChemComp-HOH:
WATER

Source
  • streptomyces globisporus (bacteria)
  • streptomyces carzinostaticus subsp. neocarzinostaticus (bacteria)
KeywordsStructural Genomics / Unknown Function / PSI-Biology / Midwest Center for Structural Genomics / MCSG / Enzyme Discovery for Natural Product Biosynthesis / NatPro / alpha-beta sandwich / neocarzinostatin biosynthesis / unknown funciton

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