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Structure paper

TitleThe mechanism of RNA 5' capping with NAD(+), NADH and desphospho-CoA.
Journal, issue, pagesNature, Vol. 535, Page 444-447, Year 2016
Publish dateAug 7, 2015 (structure data deposition date)
AuthorsBird, J.G. / Zhang, Y. / Tian, Y. / Panova, N. / Barvik, I. / Greene, L. / Liu, M. / Buckley, B. / Krasny, L. / Lee, J.K. ...Bird, J.G. / Zhang, Y. / Tian, Y. / Panova, N. / Barvik, I. / Greene, L. / Liu, M. / Buckley, B. / Krasny, L. / Lee, J.K. / Kaplan, C.D. / Ebright, R.H. / Nickels, B.E.
External linksNature / PubMed:27383794
MethodsX-ray diffraction
Resolution3 - 3.28 Å
Structure data

PDB-5d4c:
Crystal structure of Thermus thermophilus product complex for transcription initiation with ATP and CTP
Method: X-RAY DIFFRACTION / Resolution: 3.28 Å

PDB-5d4d:
Crystal structure of Thermus thermophilus product complex for transcription initiation with NAD and CTP
Method: X-RAY DIFFRACTION / Resolution: 3 Å

PDB-5d4e:
Crystal structure of Thermus thermophilus product complex for transcription initiation with 3'-dephosphate-CoA and CTP
Method: X-RAY DIFFRACTION / Resolution: 3.08 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-ZN:
Unknown entry

ChemComp-C5P:
CYTIDINE-5'-MONOPHOSPHATE

ChemComp-CTP:
CYTIDINE-5'-TRIPHOSPHATE

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM

ChemComp-HOH:
WATER

ChemComp-C:
CYTIDINE-5'-MONOPHOSPHATE

ChemComp-NAD:
NICOTINAMIDE-ADENINE-DINUCLEOTIDE / NAD*YM

ChemComp-AMP:
ADENOSINE MONOPHOSPHATE / AMP*YM

ChemComp-DPO:
DIPHOSPHATE

ChemComp-COD:
DEPHOSPHO COENZYME A

Source
  • thermus thermophilus (strain hb8 / atcc 27634 / dsm 579) (bacteria)
  • thermus thermophilus (bacteria)
  • thermus thermophilus (strain hb27 / atcc baa-163 / dsm 7039) (bacteria)
Keywordstranscription/dna / RNA polymerase / transcription initiation / bacterial / NCIN / non-canonical initiating nucleotide / primer-dependent initiation / DNA / Single-Stranded / DNA-Directed RNA Polymerases / Gene Expression Regulation / Promoter Regions / Genetic / Protein Conformation / Sigma Factor / Thermus thermophilus / TRANSCRIPTION / transcription-dna complex / Coenzyme A / 3'-dephosphate-coenzyme A

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