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TitleDetection of Active Mammalian GH31 alpha-Glucosidases in Health and Disease Using In-Class, Broad-Spectrum Activity-Based Probes.
Journal, issue, pagesAcs Cent. Sci., Vol. 2, Page 351-358, Year 2016
Publish dateFeb 8, 2016 (structure data deposition date)
AuthorsJiang, J. / Kuo, C.L. / Wu, L. / Franke, C. / Kallemeijn, W.W. / Florea, B.I. / van Meel, E. / van der Marel, G.A. / Codee, J.D. / Boot, R.G. ...Jiang, J. / Kuo, C.L. / Wu, L. / Franke, C. / Kallemeijn, W.W. / Florea, B.I. / van Meel, E. / van der Marel, G.A. / Codee, J.D. / Boot, R.G. / Davies, G.J. / Overkleeft, H.S. / Aerts, J.M.
External linksAcs Cent. Sci. / PubMed:27280170
MethodsX-ray diffraction
Resolution1.85 - 1.95 Å
Structure data

PDB-5i23:
Crystal Structure of Agd31B, alpha-transglucosylase in Glycoside Hydrolase Family 31, in complex with Cyclophellitol Aziridine probe CF022
Method: X-RAY DIFFRACTION / Resolution: 1.95 Å

PDB-5i24:
Crystal Structure of Agd31B, alpha-transglucosylase in Glycoside Hydrolase Family 31, in complex with Cyclophellitol Aziridine probe CF021
Method: X-RAY DIFFRACTION / Resolution: 1.85 Å

Chemicals

ChemComp-PG4:
TETRAETHYLENE GLYCOL / precipitant*YM

ChemComp-SO4:
SULFATE ION

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-66V:
1-(8-{[(1S,2R,3S,4S,5R,6R)-2,3,4,5-tetrahydroxy-6-(hydroxymethyl)cyclohexyl]amino}octyl)triaza-1,2-dien-2-ium

ChemComp-HOH:
WATER

ChemComp-66U:
(1R,2S,3S,4R,5S,6R)-5-amino-6-(hydroxymethyl)cyclohexane-1,2,3,4-tetrol

ChemComp-TRS:
2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL / pH buffer*YM

Source
  • cellvibrio japonicus (strain ueda107) (bacteria)
KeywordsTRANSFERASE / Alpha glycosidase / Cyclophellitol Aziridine / Inhibitor / Probe / HYDROLASE

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