EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Activation of NMDA receptors and the mechanism of inhibition by ifenprodil.
ジャーナル・号・ページ	Nature, Vol. 534, Issue 7605, Page 63-68, Year 2016
掲載日	2016年6月2日
著者	Nami Tajima / Erkan Karakas / Timothy Grant / Noriko Simorowski / Ruben Diaz-Avalos / Nikolaus Grigorieff / Hiro Furukawa /
PubMed 要旨	The physiology of N-methyl-d-aspartate (NMDA) receptors is fundamental to brain development and function. NMDA receptors are ionotropic glutamate receptors that function as heterotetramers composed ...The physiology of N-methyl-d-aspartate (NMDA) receptors is fundamental to brain development and function. NMDA receptors are ionotropic glutamate receptors that function as heterotetramers composed mainly of GluN1 and GluN2 subunits. Activation of NMDA receptors requires binding of neurotransmitter agonists to a ligand-binding domain (LBD) and structural rearrangement of an amino-terminal domain (ATD). Recent crystal structures of GluN1-GluN2B NMDA receptors bound to agonists and an allosteric inhibitor, ifenprodil, represent the allosterically inhibited state. However, how the ATD and LBD move to activate the NMDA receptor ion channel remains unclear. Here we applied X-ray crystallography, single-particle electron cryomicroscopy and electrophysiology to rat NMDA receptors to show that, in the absence of ifenprodil, the bi-lobed structure of GluN2 ATD adopts an open conformation accompanied by rearrangement of the GluN1-GluN2 ATD heterodimeric interface, altering subunit orientation in the ATD and LBD and forming an active receptor conformation that gates the ion channel.
リンク	Nature / PubMed:27135925 / PubMed Central
手法	EM (単粒子) / X線回折
解像度	2.9 - 6.8 Å
構造データ	3352 5fxg EMDB-3352: Activation of NMDA receptors and the mechanism of inhibition by ifenprodil - Active confirmation PDB-5fxg: GLUN1B-GLUN2B NMDA RECEPTOR IN ACTIVE CONFORMATION 手法: EM (単粒子) / 解像度: 6.8 Å 3353 5fxh EMDB-3353: Activation of NMDA receptors and the mechanism of inhibition by ifenprodil - Non-Active 1 Conformation PDB-5fxh: GluN1b-GluN2B NMDA receptor in non-active-1 conformation 手法: EM (単粒子) / 解像度: 5.0 Å 3354 5fxi EMDB-3354: Activation of NMDA receptors and the mechanism of inhibition by ifenprodil - Non-Active 2 confirmation PDB-5fxi: GluN1b-GluN2B NMDA receptor structure in non-active-2 conformation 手法: EM (単粒子) / 解像度: 5.5 Å 3355 5fxj EMDB-3355: Activation of NMDA receptors and the mechanism of inhibition by ifenprodil - Class X PDB-5fxj: GluN1b-GluN2B NMDA receptor structure-Class X 手法: EM (単粒子) / 解像度: 6.25 Å 3356 5fxk EMDB-3356: Activation of NMDA receptors and the mechanism of inhibition by ifenprodil - Class Y PDB-5fxk: GluN1b-GluN2B NMDA receptor structure-Class Y 手法: EM (単粒子) / 解像度: 6.25 Å PDB-5b3j: Activation of NMDA receptors and the mechanism of inhibition by ifenprodil 手法: X-RAY DIFFRACTION / 解像度: 2.9 Å
化合物	ChemComp-NA: Unknown entry / ナトリウムカチオン ChemComp-HOH: WATER
由来	rattus norvegicus (ドブネズミ) xenopus laevis (アフリカツメガエル) mus musculus (ハツカネズミ)
キーワード	TRANSPORT PROTEIN / NMDA receptor / SIGNALING PROTEIN / GLUTAMATE RECEPTOR / GLUN1 / GLUN2B / ION CHANNEL