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-Structure paper
Title | Supramolecular organization of the human N-BAR domain in shaping the sarcolemma membrane. |
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Journal, issue, pages | J Struct Biol, Vol. 194, Issue 3, Page 375-382, Year 2016 |
Publish date | Mar 22, 2016 |
Authors | Bertram Daum / Andrea Auerswald / Tobias Gruber / Gerd Hause / Jochen Balbach / Werner Kühlbrandt / Annette Meister / |
PubMed Abstract | The 30kDa N-BAR domain of the human Bin1 protein is essential for the generation of skeletal muscle T-tubules. By electron cryo-microscopy and electron cryo-tomography with a direct electron ...The 30kDa N-BAR domain of the human Bin1 protein is essential for the generation of skeletal muscle T-tubules. By electron cryo-microscopy and electron cryo-tomography with a direct electron detector, we found that Bin1-N-BAR domains assemble into scaffolds of low long-range order that form flexible membrane tubules. The diameter of the tubules closely matches the curved shape of the N-BAR domain, which depends on the composition of the target membrane. These insights are fundamental to our understanding of T-tubule formation and function in human skeletal muscle. |
External links | J Struct Biol / PubMed:27016283 |
Methods | EM (subtomogram averaging) |
Resolution | 36.0 - 50.0 Å |
Structure data | EMDB-8126: EMDB-8127: |
Source |
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