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Structure paper

TitleStructural Basis for the Altered PAM Specificities of Engineered CRISPR-Cas9
Journal, issue, pagesMol. Cell, Vol. 61, Page 886-894, Year 2016
Publish dateFeb 2, 2016 (structure data deposition date)
AuthorsHirano, S. / Nishimasu, H. / Ishitani, R. / Nureki, O.
External linksMol. Cell / PubMed:26990991
MethodsX-ray diffraction
Resolution2 - 2.2 Å
Structure data

PDB-5b2r:
Crystal structure of the Streptococcus pyogenes Cas9 VQR variant in complex with sgRNA and target DNA (TGA PAM)
Method: X-RAY DIFFRACTION / Resolution: 2 Å

PDB-5b2s:
Crystal structure of the Streptococcus pyogenes Cas9 EQR variant in complex with sgRNA and target DNA (TGAG PAM)
Method: X-RAY DIFFRACTION / Resolution: 2.2 Å

PDB-5b2t:
Crystal structure of the Streptococcus pyogenes Cas9 VRER variant in complex with sgRNA and target DNA (TGCG PAM)
Method: X-RAY DIFFRACTION / Resolution: 2.2 Å

Chemicals

ChemComp-K:
Unknown entry

ChemComp-MG:
Unknown entry

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-ACT:
ACETATE ION

ChemComp-HOH:
WATER

Source
  • streptococcus pyogenes serotype m1 (bacteria)
  • streptococcus pyogenes (bacteria)
KeywordsHYDROLASE/RNA/DNA / CRISPR-Cas9 / genome engineering / HYDROLASE-RNA-DNA complex

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