Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Structural Basis for Recognition of Human Enterovirus 71 by a Bivalent Broadly Neutralizing Monoclonal Antibody.
Journal, issue, pages	PLoS Pathog, Vol. 12, Issue 3, Page e1005454, Year 2016
Publish date	Mar 3, 2016
Authors	Xiaohua Ye / Chen Fan / Zhiqiang Ku / Teng Zuo / Liangliang Kong / Chao Zhang / Jinping Shi / Qingwei Liu / Tan Chen / Yingyi Zhang / Wen Jiang / Linqi Zhang / Zhong Huang / Yao Cong /
PubMed Abstract	Enterovirus 71 (EV71) is the main pathogen responsible for hand, foot and mouth disease with severe neurological complications and even death in young children. We have recently identified a highly ...Enterovirus 71 (EV71) is the main pathogen responsible for hand, foot and mouth disease with severe neurological complications and even death in young children. We have recently identified a highly potent anti-EV71 neutralizing monoclonal antibody, termed D5. Here we investigated the structural basis for recognition of EV71 by the antibody D5. Four three-dimensional structures of EV71 particles in complex with IgG or Fab of D5 were reconstructed by cryo-electron microscopy (cryo-EM) single particle analysis all at subnanometer resolutions. The most critical EV71 mature virion-Fab structure was resolved to a resolution of 4.8 Å, which is rare in cryo-EM studies of virus-antibody complex so far. The structures reveal a bivalent binding pattern of D5 antibody across the icosahedral 2-fold axis on mature virion, suggesting that D5 binding may rigidify virions to prevent their conformational changes required for subsequent RNA release. Moreover, we also identified that the complementary determining region 3 (CDR3) of D5 heavy chain directly interacts with the extremely conserved VP1 GH-loop of EV71, which was validated by biochemical and virological assays. We further showed that D5 is indeed able to neutralize a variety of EV71 genotypes and strains. Moreover, D5 could potently confer protection in a mouse model of EV71 infection. Since the conserved VP1 GH-loop is involved in EV71 binding with its uncoating receptor, the scavenger receptor class B, member 2 (SCARB2), the broadly neutralizing ability of D5 might attribute to its inhibition of EV71 from binding SCARB2. Altogether, our results elucidate the structural basis for the binding and neutralization of EV71 by the broadly neutralizing antibody D5, thereby enhancing our understanding of antibody-based protection against EV71 infection.
External links	PLoS Pathog / PubMed:26938634 / PubMed Central
Methods	EM (single particle)
Resolution	4.8 - 7.2 Å
Structure data	6365 3jau EMDB-6365: The cryoEM map of EV71 mature viron in complex with intact antibody D5 PDB-3jau: The cryoEM map of EV71 mature viron in complex with the Fab fragment of antibody D5 Method: EM (single particle) / Resolution: 7.2 Å 6366 3jau EMDB-6366, PDB-3jau: The cryoEM map of EV71 mature viron in complex with the Fab fragment of antibody D5 Method: EM (single particle) / Resolution: 4.8 Å 6383 3jau EMDB-6383: The cryoEM map of EV71 empty particle in complex with the Fab fragment of antibody D5 PDB-3jau: The cryoEM map of EV71 mature viron in complex with the Fab fragment of antibody D5 Method: EM (single particle) / Resolution: 6.0 Å 6384 3jau EMDB-6384: The cryoEM map of EV71 VLP in complex with intact antibody D5 PDB-3jau: The cryoEM map of EV71 mature viron in complex with the Fab fragment of antibody D5 Method: EM (single particle) / Resolution: 5.5 Å
Source	Homo sapiens (human) human enterovirus mus musculus (house mouse)
Keywords	VIRUS/IMMUNE SYSTEM / Enterovirus 71(EV71) / virus-antibody complex / bivalent binding / high resolution cryo-EM / VIRUS-IMMUNE SYSTEM complex