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-Structure paper
Title | Structure of a Bacterial Virus DNA-Injection Protein Complex Reveals a Decameric Assembly with a Constricted Molecular Channel. |
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Journal, issue, pages | PLoS One, Vol. 11, Issue 2, Page e0149337, Year 2016 |
Publish date | Feb 16, 2016 |
Authors | Haiyan Zhao / Jeffrey A Speir / Tsutomu Matsui / Zihan Lin / Lingfei Liang / Anna Y Lynn / Brittany Varnado / Thomas M Weiss / Liang Tang / |
PubMed Abstract | The multi-layered cell envelope structure of Gram-negative bacteria represents significant physical and chemical barriers for short-tailed phages to inject phage DNA into the host cytoplasm. Here we ...The multi-layered cell envelope structure of Gram-negative bacteria represents significant physical and chemical barriers for short-tailed phages to inject phage DNA into the host cytoplasm. Here we show that a DNA-injection protein of bacteriophage Sf6, gp12, forms a 465-kDa, decameric assembly in vitro. The electron microscopic structure of the gp12 assembly shows a ~150-Å, mushroom-like architecture consisting of a crown domain and a tube-like domain, which embraces a 25-Å-wide channel that could precisely accommodate dsDNA. The constricted channel suggests that gp12 mediates rapid, uni-directional injection of phage DNA into host cells by providing a molecular conduit for DNA translocation. The assembly exhibits a 10-fold symmetry, which may be a common feature among DNA-injection proteins of P22-like phages and may suggest a symmetry mismatch with respect to the 6-fold symmetric phage tail. The gp12 monomer is highly flexible in solution, supporting a mechanism for translocation of the protein through the conduit of the phage tail toward the host cell envelope, where it assembles into a DNA-injection device. |
External links | PLoS One / PubMed:26882199 / PubMed Central |
Methods | EM (single particle) |
Resolution | 11.0 Å |
Structure data | EMDB-6330: |
Source |
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