Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Cryo-EM structure of the yeast U4/U6.U5 tri-snRNP at 3.7 Å resolution.
Journal, issue, pages	Nature, Vol. 530, Issue 7590, Page 298-302, Year 2016
Publish date	Feb 18, 2016
Authors	Thi Hoang Duong Nguyen / Wojciech P Galej / Xiao-Chen Bai / Chris Oubridge / Andrew J Newman / Sjors H W Scheres / Kiyoshi Nagai /
PubMed Abstract	U4/U6.U5 tri-snRNP represents a substantial part of the spliceosome before activation. A cryo-electron microscopy structure of Saccharomyces cerevisiae U4/U6.U5 tri-snRNP at 3.7 Å resolution led ...U4/U6.U5 tri-snRNP represents a substantial part of the spliceosome before activation. A cryo-electron microscopy structure of Saccharomyces cerevisiae U4/U6.U5 tri-snRNP at 3.7 Å resolution led to an essentially complete atomic model comprising 30 proteins plus U4/U6 and U5 small nuclear RNAs (snRNAs). The structure reveals striking interweaving interactions of the protein and RNA components, including extended polypeptides penetrating into subunit interfaces. The invariant ACAGAGA sequence of U6 snRNA, which base-pairs with the 5'-splice site during catalytic activation, forms a hairpin stabilized by Dib1 and Prp8 while the adjacent nucleotides interact with the exon binding loop 1 of U5 snRNA. Snu114 harbours GTP, but its putative catalytic histidine is held away from the γ-phosphate by hydrogen bonding to a tyrosine in the amino-terminal domain of Prp8. Mutation of this histidine to alanine has no detectable effect on yeast growth. The structure provides important new insights into the spliceosome activation process leading to the formation of the catalytic centre.
External links	Nature / PubMed:26829225 / PubMed Central
Methods	EM (single particle)
Resolution	3.6 - 4.6 Å
Structure data	EMDB-8006: The open state of the yeast U4/U6.U5 tri-snRNP at 4.2 Angstrom Method: EM (single particle) / Resolution: 4.2 Å EMDB-8007: The closed state of the yeast spliceosomal U4/U6.U5 tri-snRNP at 4.3 Angstrom Method: EM (single particle) / Resolution: 4.3 Å EMDB-8008: Combined Body and arm regions of the yeast spliceosomal U4/U6.U5 tri-snRNP by masked classification and refinement-Class 1 Method: EM (single particle) / Resolution: 4.6 Å EMDB-8009: Combined Body and arm regions of the yeast spliceosomal U4/U6.U5 tri-snRNP by masked classification and refinement-Class 2 Method: EM (single particle) / Resolution: 4.5 Å EMDB-8010: Combined Body and arm regions of the yeast spliceosomal U4/U6.U5 tri-snRNP by masked classification and refinement-Class 3 Method: EM (single particle) / Resolution: 4.5 Å 8011 5gam EMDB-8011, PDB-5gam: Foot region of the yeast spliceosomal U4/U6.U5 tri-snRNP Method: EM (single particle) / Resolution: 3.7 Å 8012 5gan EMDB-8012, PDB-5gan: The overall structure of the yeast spliceosomal U4/U6.U5 tri-snRNP at 3.7 Angstrom Method: EM (single particle) / Resolution: 3.7 Å 8013 5gao EMDB-8013, PDB-5gao: Head region of the yeast spliceosomal U4/U6.U5 tri-snRNP Method: EM (single particle) / Resolution: 4.2 Å 8014 5gap EMDB-8014, PDB-5gap: Body region of the U4/U6.U5 tri-snRNP Method: EM (single particle) / Resolution: 3.6 Å
Chemicals	ChemComp-GTP: GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM
Source	saccharomyces cerevisiae (brewer's yeast)
Keywords	TRANSCRIPTION / pre-mRNA splicing / snRNP / GTPase / U5 snRNA / Prp8 / spliceosome / U4/U6 snRNP / Brr2 / Snu114 / RNA-protein complex / U4/U6.U5 snRNP