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TitleEffects of Active-Site Modification and Quaternary Structure on the Regioselectivity of Catechol-O-Methyltransferase.
Journal, issue, pagesAngew. Chem. Int. Ed. Engl., Vol. 55, Page 2683-2687, Year 2016
Publish dateDec 22, 2015 (structure data deposition date)
AuthorsLaw, B.J. / Bennett, M.R. / Thompson, M.L. / Levy, C. / Shepherd, S.A. / Leys, D. / Micklefield, J.
External linksAngew. Chem. Int. Ed. Engl. / PubMed:26797714
MethodsX-ray diffraction
Resolution1.63 Å
Structure data

PDB-5fhq:
Crystal structure of (WT) Rat Catechol-O-Methyltransferase in complex with AdoMet and 3,5-dinitrocatechol (DNC)
Method: X-RAY DIFFRACTION / Resolution: 1.63 Å

PDB-5fhr:
Crystal structure of Y200L mutant of Rat Catechol-O-Methyltransferase in complex with AdoMet and 3,5-dinitrocatechol
Method: X-RAY DIFFRACTION / Resolution: 1.63 Å

Chemicals

ChemComp-SAM:
S-ADENOSYLMETHIONINE

ChemComp-DNC:
3,5-DINITROCATECHOL

ChemComp-MG:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-BR:
BROMIDE ION

Source
  • rattus norvegicus (Norway rat)
KeywordsTRANSFERASE / Methyltransferase Regioselectivity

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