Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	The 3.8 Å structure of the U4/U6.U5 tri-snRNP: Insights into spliceosome assembly and catalysis.
Journal, issue, pages	Science, Vol. 351, Issue 6272, Page 466-475, Year 2016
Publish date	Jan 29, 2016
Authors	Ruixue Wan / Chuangye Yan / Rui Bai / Lin Wang / Min Huang / Catherine C L Wong / Yigong Shi /
PubMed Abstract	Splicing of precursor messenger RNA is accomplished by a dynamic megacomplex known as the spliceosome. Assembly of a functional spliceosome requires a preassembled U4/U6.U5 tri-snRNP complex, which ...Splicing of precursor messenger RNA is accomplished by a dynamic megacomplex known as the spliceosome. Assembly of a functional spliceosome requires a preassembled U4/U6.U5 tri-snRNP complex, which comprises the U5 small nuclear ribonucleoprotein (snRNP), the U4 and U6 small nuclear RNA (snRNA) duplex, and a number of protein factors. Here we report the three-dimensional structure of a Saccharomyces cerevisiae U4/U6.U5 tri-snRNP at an overall resolution of 3.8 angstroms by single-particle electron cryomicroscopy. The local resolution for the core regions of the tri-snRNP reaches 3.0 to 3.5 angstroms, allowing construction of a refined atomic model. Our structure contains U5 snRNA, the extensively base-paired U4/U6 snRNA, and 30 proteins including Prp8 and Snu114, which amount to 8495 amino acids and 263 nucleotides with a combined molecular mass of ~1 megadalton. The catalytic nucleotide U80 from U6 snRNA exists in an inactive conformation, stabilized by its base-pairing interactions with U4 snRNA and protected by Prp3. Pre-messenger RNA is bound in the tri-snRNP through base-pairing interactions with U6 snRNA and loop I of U5 snRNA. This structure, together with that of the spliceosome, reveals the molecular choreography of the snRNAs in the activation process of the spliceosomal ribozyme.
External links	Science / PubMed:26743623
Methods	EM (single particle)
Resolution	3.46 - 7.9 Å
Structure data	6561 3jcm EMDB-6561: The cryo-EM structure of yeast spliceosomal U4/U6.U5 tri-snRNP at 3.8 A PDB-3jcm: Cryo-EM structure of the spliceosomal U4/U6.U5 tri-snRNP Method: EM (single particle) / Resolution: 3.81 Å 6562 3jcm EMDB-6562: The cryo-EM structure of yeast spliceosomal U4/U6.U5 tri-snRNP (improved map for U4/U6 and U5 RNA region at 3.75 A) PDB-3jcm: Cryo-EM structure of the spliceosomal U4/U6.U5 tri-snRNP Method: EM (single particle) / Resolution: 3.75 Å 6563 3jcm EMDB-6563: The cryo-EM structure of yeast spliceosomal U4/U6.U5 tri-snRNP (improved map for U4/U6 RNA region at 3.57 A) PDB-3jcm: Cryo-EM structure of the spliceosomal U4/U6.U5 tri-snRNP Method: EM (single particle) / Resolution: 3.57 Å 6564 3jcm EMDB-6564: The cryo-EM structure of yeast spliceosomal U4/U6.U5 tri-snRNP (improved map for Prp31 region at 3.52 A) PDB-3jcm: Cryo-EM structure of the spliceosomal U4/U6.U5 tri-snRNP Method: EM (single particle) / Resolution: 3.52 Å 6565 3jcm EMDB-6565: The cryo-EM structure of yeast spliceosomal U4/U6.U5 tri-snRNP (improved map for Prp8 region at 3.49 A) PDB-3jcm: Cryo-EM structure of the spliceosomal U4/U6.U5 tri-snRNP Method: EM (single particle) / Resolution: 3.49 Å 6566 3jcm EMDB-6566: The cryo-EM structure of yeast spliceosomal U4/U6.U5 tri-snRNP (improved map for Prp3 region at 3.46 A) PDB-3jcm: Cryo-EM structure of the spliceosomal U4/U6.U5 tri-snRNP Method: EM (single particle) / Resolution: 3.46 Å 6567 3jcm EMDB-6567: The cryo-EM structure of yeast spliceosomal U4/U6.U5 tri-snRNP (improved map for Prp4 region at 3.61 A) PDB-3jcm: Cryo-EM structure of the spliceosomal U4/U6.U5 tri-snRNP Method: EM (single particle) / Resolution: 3.61 Å 6568 3jcm EMDB-6568: The cryo-EM structure of yeast spliceosomal U4/U6.U5 tri-snRNP (improved map for Prp4 WD40 region at 3.70 A) PDB-3jcm: Cryo-EM structure of the spliceosomal U4/U6.U5 tri-snRNP Method: EM (single particle) / Resolution: 3.7 Å 6569 3jcm EMDB-6569: The cryo-EM structure of yeast spliceosomal U4/U6.U5 tri-snRNP (improved map for U5 RNA region at 3.70 A) PDB-3jcm: Cryo-EM structure of the spliceosomal U4/U6.U5 tri-snRNP Method: EM (single particle) / Resolution: 3.77 Å 6570 3jcm EMDB-6570: The cryo-EM structure of yeast spliceosomal U4/U6.U5 tri-snRNP (improved map for U5 Snu114 region at 3.77 A) PDB-3jcm: Cryo-EM structure of the spliceosomal U4/U6.U5 tri-snRNP Method: EM (single particle) / Resolution: 3.77 Å 6571 3jcm EMDB-6571: The cryo-EM structure of yeast spliceosomal U4/U6.U5 tri-snRNP (improved map for U5 Sm ring region at 4.7 A) PDB-3jcm: Cryo-EM structure of the spliceosomal U4/U6.U5 tri-snRNP Method: EM (single particle) / Resolution: 4.7 Å 6572 3jcm EMDB-6572: The cryo-EM structure of yeast spliceosomal U4/U6.U5 tri-snRNP (improved map for U5 Brr2 region at 7.9 A) PDB-3jcm: Cryo-EM structure of the spliceosomal U4/U6.U5 tri-snRNP Method: EM (single particle) / Resolution: 7.9 Å 6573 3jcm EMDB-6573: The cryo-EM structure of yeast spliceosomal U4/U6.U5 tri-snRNP (improved map for pre-mRNA region at 3.60 A) PDB-3jcm: Cryo-EM structure of the spliceosomal U4/U6.U5 tri-snRNP Method: EM (single particle) / Resolution: 3.6 Å
Chemicals	ChemComp-GTP: GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying moleculeYM / Guanosine triphosphate ChemComp-M7M*: N,N,7-trimethylguanosine 5'-(trihydrogen diphosphate)
Source	Saccharomyces cerevisiae (brewer's yeast) saccharomyces cerevisiae s288c (yeast) synthetic construct (others)
Keywords	TRANSCRIPTION / U4/U6.U5 tri-snRNP / pre-mRNA