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Structure paper

TitleEngineering an allosteric transcription factor to respond to new ligands.
Journal, issue, pagesNat. Methods, Vol. 13, Page 177-183, Year 2016
Publish dateDec 24, 2014 (structure data deposition date)
AuthorsTaylor, N.D. / Garruss, A.S. / Moretti, R. / Chan, S. / Arbing, M.A. / Cascio, D. / Rogers, J.K. / Isaacs, F.J. / Kosuri, S. / Baker, D. ...Taylor, N.D. / Garruss, A.S. / Moretti, R. / Chan, S. / Arbing, M.A. / Cascio, D. / Rogers, J.K. / Isaacs, F.J. / Kosuri, S. / Baker, D. / Fields, S. / Church, G.M. / Raman, S.
External linksNat. Methods / PubMed:26689263
MethodsX-ray diffraction
Resolution2.71 - 3.1 Å
Structure data

PDB-4rzs:
Lac repressor engineered to bind sucralose, unliganded tetramer
Method: X-RAY DIFFRACTION / Resolution: 2.71 Å

PDB-4rzt:
Lac repressor engineered to bind sucralose, sucralose-bound tetramer
Method: X-RAY DIFFRACTION / Resolution: 3.1 Å

Chemicals

ChemComp-GOL:
GLYCEROL

ChemComp-HOH:
WATER

Source
  • escherichia coli (E. coli)
KeywordsTRANSCRIPTION / sucralose / lacI / lac repressor / lactose operon repressor / protein design / allostery / Escherichia coli

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