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Structure paper

TitleSeeding for sirtuins: microseed matrix seeding to obtain crystals of human Sirt3 and Sirt2 suitable for soaking.
Journal, issue, pagesActa Crystallogr. ,Sect. F, Vol. 71, Page 1498-1510, Year 2015
Publish dateAug 14, 2015 (structure data deposition date)
AuthorsRumpf, T. / Gerhardt, S. / Einsle, O. / Jung, M.
External linksActa Crystallogr. ,Sect. F / PubMed:26625292
MethodsX-ray diffraction
Resolution1.63 - 2.01 Å
Structure data

PDB-5d7n:
Crystal structure of human Sirt3 at an improved resolution
Method: X-RAY DIFFRACTION / Resolution: 1.83 Å

PDB-5d7o:
Crystal structure of Sirt2-ADPR at an improved resolution
Method: X-RAY DIFFRACTION / Resolution: 1.63 Å

PDB-5d7p:
Crystal structure of human Sirt2 in complex with ADPR and EX-243
Method: X-RAY DIFFRACTION / Resolution: 1.76 Å

PDB-5d7q:
Crystal structure of human Sirt2 in complex with ADPR and CHIC35
Method: X-RAY DIFFRACTION / Resolution: 2.01 Å

Chemicals

ChemComp-ZN:
Unknown entry

ChemComp-PEG:
DI(HYDROXYETHYL)ETHER

ChemComp-MG:
Unknown entry

ChemComp-1PE:
PENTAETHYLENE GLYCOL / precipitant*YM

ChemComp-GOL:
GLYCEROL

ChemComp-PGE:
TRIETHYLENE GLYCOL

ChemComp-PG4:
TETRAETHYLENE GLYCOL / precipitant*YM

ChemComp-HOH:
WATER

ChemComp-AR6:
[(2R,3S,4R,5R)-5-(6-AMINOPURIN-9-YL)-3,4-DIHYDROXY-OXOLAN-2-YL]METHYL

ChemComp-OCZ:
(1S)-6-chloro-2,3,4,9-tetrahydro-1H-carbazole-1- carboxamide

ChemComp-4I5:
(6S)-2-chloro-5,6,7,8,9,10-hexahydrocyclohepta[b]indole-6-carboxamide

Source
  • homo sapiens (human)
KeywordsHYDROLASE / Sirtuin 3 / Deacylase / Human Sirt2 / Human Sirtuin 2 / Inhibitor complex

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