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TitleAn O-Methyltransferase Is Required for Infection of Tick Cells by Anaplasma phagocytophilum.
Journal, issue, pagesPlos Pathog., Vol. 11, Page e1005248-e1005248, Year 2015
Publish dateJan 3, 2014 (structure data deposition date)
AuthorsOliva Chavez, A.S. / Fairman, J.W. / Felsheim, R.F. / Nelson, C.M. / Herron, M.J. / Higgins, L. / Burkhardt, N.Y. / Oliver, J.D. / Markowski, T.W. / Kurtti, T.J. ...Oliva Chavez, A.S. / Fairman, J.W. / Felsheim, R.F. / Nelson, C.M. / Herron, M.J. / Higgins, L. / Burkhardt, N.Y. / Oliver, J.D. / Markowski, T.W. / Kurtti, T.J. / Edwards, T.E. / Munderloh, U.G.
External linksPlos Pathog. / PubMed:26544981
MethodsX-ray diffraction
Resolution1.5 - 2.3 Å
Structure data

PDB-4oa5:
X-ray crystal structure of an O-methyltransferase from Anaplasma phagocytophilum bound to SAH solved by iodide SAD phasing
Method: X-RAY DIFFRACTION / Resolution: 2.3 Å

PDB-4oa8:
X-ray crystal structure of O-methyltransferase from Anaplasma phagocytophilum in apo form
Method: X-RAY DIFFRACTION / Resolution: 2.15 Å

PDB-4pca:
X-ray crystal structure of an O-methyltransferase from Anaplasma phagocytophilum bound to SAH and Manganese
Method: X-RAY DIFFRACTION / Resolution: 1.5 Å

PDB-4pcl:
X-ray crystal structure of an O-methyltransferase from Anaplasma phagocytophilum bound to SAM and a Manganese ion.
Method: X-RAY DIFFRACTION / Resolution: 1.85 Å

Chemicals

ChemComp-SAH:
S-ADENOSYL-L-HOMOCYSTEINE

ChemComp-IOD:
IODIDE ION

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-GOL:
GLYCEROL

ChemComp-HOH:
WATER

ChemComp-NA:
Unknown entry

ChemComp-MN:
Unknown entry

ChemComp-SAM:
S-ADENOSYLMETHIONINE

Source
  • anaplasma phagocytophilum (agent of human granulocytic ehrlichiosis)
  • anaplasma phagocytophilum str. hge1 (bacteria)
KeywordsTRANSFERASE / Structural Genomics / NIAID / National Institute of Allergy and Infectious Diseases / Seattle Structural Genomics Center for Infectious Disease / SSGCID / O-methyltransferase / methyltransferase SAH / O-methyltransferase SAM mangenese

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