EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	In situ structures of the segmented genome and RNA polymerase complex inside a dsRNA virus.
ジャーナル・号・ページ	Nature, Vol. 527, Issue 7579, Page 531-534, Year 2015
掲載日	2015年11月26日
著者	Xing Zhang / Ke Ding / Xuekui Yu / Winston Chang / Jingchen Sun / Z Hong Zhou /
PubMed 要旨	Viruses in the Reoviridae, like the triple-shelled human rotavirus and the single-shelled insect cytoplasmic polyhedrosis virus (CPV), all package a genome of segmented double-stranded RNAs (dsRNAs) ...Viruses in the Reoviridae, like the triple-shelled human rotavirus and the single-shelled insect cytoplasmic polyhedrosis virus (CPV), all package a genome of segmented double-stranded RNAs (dsRNAs) inside the viral capsid and carry out endogenous messenger RNA synthesis through a transcriptional enzyme complex (TEC). By direct electron-counting cryoelectron microscopy and asymmetric reconstruction, we have determined the organization of the dsRNA genome inside quiescent CPV (q-CPV) and the in situ atomic structures of TEC within CPV in both quiescent and transcribing (t-CPV) states. We show that the ten segmented dsRNAs in CPV are organized with ten TECs in a specific, non-symmetric manner, with each dsRNA segment attached directly to a TEC. The TEC consists of two extensively interacting subunits: an RNA-dependent RNA polymerase (RdRP) and an NTPase VP4. We find that the bracelet domain of RdRP undergoes marked conformational change when q-CPV is converted to t-CPV, leading to formation of the RNA template entry channel and access to the polymerase active site. An amino-terminal helix from each of two subunits of the capsid shell protein (CSP) interacts with VP4 and RdRP. These findings establish the link between sensing of environmental cues by the external proteins and activation of endogenous RNA transcription by the TEC inside the virus.
リンク	Nature / PubMed:26503045 / PubMed Central
手法	EM (単粒子)
解像度	3.3 - 22.0 Å
構造データ	6404 3jb7 EMDB-6404, PDB-3jb7: In situ structures of the segmented genome and RNA polymerase complex inside a dsRNA virus 手法: EM (単粒子) / 解像度: 4.0 Å EMDB-6405: In situ structures of the segmented genome and RNA polymerase complex inside a dsRNA virus 手法: EM (単粒子) / 解像度: 4.0 Å EMDB-6406: In situ structures of the segmented genome and RNA polymerase complex inside a dsRNA virus 手法: EM (単粒子) / 解像度: 3.3 Å EMDB-6407: In situ structures of the segmented genome and RNA polymerase complex inside a dsRNA virus 手法: EM (単粒子) / 解像度: 3.3 Å 6408 3jb6 EMDB-6408, PDB-3jb6: In situ structures of the segmented genome and RNA polymerase complex inside a dsRNA virus 手法: EM (単粒子) / 解像度: 3.3 Å EMDB-6409: In situ structures of the segmented genome and RNA polymerase complex inside a dsRNA virus 手法: EM (単粒子) / 解像度: 22.0 Å
化合物	ChemComp-GTP: GUANOSINE-5'-TRIPHOSPHATE / GTP / GTP, エネルギー貯蔵分子YM ChemComp-CTP*: CYTIDINE-5'-TRIPHOSPHATE / CTP
由来	bombyx mori cypovirus 1 (ウイルス) Bombyx mori (カイコ)
キーワード	TRANSFERASE/VIRAL PROTEIN / dsRNA genome organization / viral polymerase / TRANSFERASE-VIRAL PROTEIN complex / TRANSFERASE/VIRAL PROTEIN/RNA / TRANSFERASE-VIRAL PROTEIN-RNA complex