+検索条件
-Structure paper
タイトル | Structural diversity of supercoiled DNA. |
---|---|
ジャーナル・号・ページ | Nat Commun, Vol. 6, Page 8440, Year 2015 |
掲載日 | 2015年10月12日 |
著者 | Rossitza N Irobalieva / Jonathan M Fogg / Daniel J Catanese / Thana Sutthibutpong / Muyuan Chen / Anna K Barker / Steven J Ludtke / Sarah A Harris / Michael F Schmid / Wah Chiu / Lynn Zechiedrich / |
PubMed 要旨 | By regulating access to the genetic code, DNA supercoiling strongly affects DNA metabolism. Despite its importance, however, much about supercoiled DNA (positively supercoiled DNA, in particular) ...By regulating access to the genetic code, DNA supercoiling strongly affects DNA metabolism. Despite its importance, however, much about supercoiled DNA (positively supercoiled DNA, in particular) remains unknown. Here we use electron cryo-tomography together with biochemical analyses to investigate structures of individual purified DNA minicircle topoisomers with defined degrees of supercoiling. Our results reveal that each topoisomer, negative or positive, adopts a unique and surprisingly wide distribution of three-dimensional conformations. Moreover, we uncover striking differences in how the topoisomers handle torsional stress. As negative supercoiling increases, bases are increasingly exposed. Beyond a sharp supercoiling threshold, we also detect exposed bases in positively supercoiled DNA. Molecular dynamics simulations independently confirm the conformational heterogeneity and provide atomistic insight into the flexibility of supercoiled DNA. Our integrated approach reveals the three-dimensional structures of DNA that are essential for its function. |
リンク | Nat Commun / PubMed:26455586 / PubMed Central |
手法 | EM (トモグラフィー) |
構造データ | EMDB-6462: |
由来 |
|