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TitleCryo-EM shows the polymerase structures and a nonspooled genome within a dsRNA virus.
Journal, issue, pagesScience, Vol. 349, Issue 6254, Page 1347-1350, Year 2015
Publish dateSep 18, 2015
AuthorsHongrong Liu / Lingpeng Cheng /
PubMed AbstractDouble-stranded RNA (dsRNA) viruses possess a segmented dsRNA genome and a number of RNA-dependent RNA polymerases (RdRps) enclosed in a capsid. Until now, the precise structures of genomes and RdRps ...Double-stranded RNA (dsRNA) viruses possess a segmented dsRNA genome and a number of RNA-dependent RNA polymerases (RdRps) enclosed in a capsid. Until now, the precise structures of genomes and RdRps within the capsids have been unknown. Here we report the structures of RdRps and associated RNAs within nontranscribing and transcribing cypoviruses (NCPV and TCPV, respectively), using a combination of cryo-electron microscopy (cryo-EM) and a symmetry-mismatch reconstruction method. The RdRps and associated RNAs appear to exhibit a pseudo-D3 symmetric organization in both NCPV and TCPV. However, the molecular interactions between RdRps and the genomic RNA were found to differ in these states. Our work provides insight into the mechanisms of the replication and transcription in dsRNA viruses and paves a way for structural determination of lower-symmetry complexes enclosed in higher-symmetry structures.
External linksScience / PubMed:26383954
MethodsEM (single particle)
Resolution4.8 - 12.0 Å
Structure data

6321

3ja4

EMDB-6321: Genome and RdRp structures within the capsid of transcribing cypoviruses
PDB-3ja4: RNA-dependent RNA polymerases of transcribing cypoviruses
Method: EM (single particle) / Resolution: 12.0 Å

6322

3ja5

EMDB-6322, PDB-3ja5:
Genome and RdRp structure within the capsid of no-transcribing cypovirus
Method: EM (single particle) / Resolution: 12.0 Å

Source
  • bombyx mori cypovirus 1
KeywordsTRANSFERASE / RNA-dependent RNA polymerases / transcribing cypoviruses / icosahedral virus / non-transcribing cypovirus / RNA-dependent RNA polymerase / dsRNA virus

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