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Structure paper

TitleCrystal Structure of Staphylococcus aureus Cas9.
Journal, issue, pagesCell, Vol. 162, Page 1113-1126, Year 2015
Publish dateAug 1, 2015 (structure data deposition date)
AuthorsNishimasu, H. / Cong, L. / Yan, W.X. / Ran, F.A. / Zetsche, B. / Li, Y. / Kurabayashi, A. / Ishitani, R. / Zhang, F. / Nureki, O.
External linksCell / PubMed:26317473
MethodsX-ray diffraction
Resolution2.6 - 2.7 Å
Structure data

PDB-5axw:
Crystal structure of Staphylococcus aureus Cas9 in complex with sgRNA and target DNA (TTGGGT PAM)
Method: X-RAY DIFFRACTION / Resolution: 2.7 Å

PDB-5czz:
Crystal structure of Staphylococcus aureus Cas9 in complex with sgRNA and target DNA (TTGAAT PAM)
Method: X-RAY DIFFRACTION / Resolution: 2.6 Å

Chemicals

ChemComp-NA:
Unknown entry

ChemComp-PO4:
PHOSPHATE ION

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-HOH:
WATER

Source
  • staphylococcus aureus subsp. aureus (bacteria)
  • staphylococcus aureus (bacteria)
KeywordsHYDROLASE/RNA/DNA / CRISPR-Cas9 / genome engineering / HYDROLASE-RNA-DNA complex

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