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Structure paper

TitleActive Transport of Phosphorylated Carbohydrates Promotes Intestinal Colonization and Transmission of a Bacterial Pathogen.
Journal, issue, pagesPlos Pathog., Vol. 11, Page e1005107-e1005107, Year 2015
Publish dateAug 26, 2014 (structure data deposition date)
AuthorsSit, B. / Crowley, S.M. / Bhullar, K. / Lai, C.C. / Tang, C. / Hooda, Y. / Calmettes, C. / Khambati, H. / Ma, C. / Brumell, J.H. ...Sit, B. / Crowley, S.M. / Bhullar, K. / Lai, C.C. / Tang, C. / Hooda, Y. / Calmettes, C. / Khambati, H. / Ma, C. / Brumell, J.H. / Schryvers, A.B. / Vallance, B.A. / Moraes, T.F.
External linksPlos Pathog. / PubMed:26295949
MethodsX-ray diffraction
Resolution1.278 - 1.93 Å
Structure data

PDB-4r72:
Structure of the periplasmic binding protein AfuA from Actinobacillus pleuropneumoniae (apo form)
Method: X-RAY DIFFRACTION / Resolution: 1.6 Å

PDB-4r73:
Structure of the periplasmic binding protein AfuA from Actinobacillus pleuropneumoniae (endogenous glucose-6-phosphate and mannose-6-phosphate bound)
Method: X-RAY DIFFRACTION / Resolution: 1.6 Å

PDB-4r74:
Structure of the periplasmic binding protein AfuA from Actinobacillus pleuropneumoniae (exogenous fructose-6-phosphate bound)
Method: X-RAY DIFFRACTION / Resolution: 1.93 Å

PDB-4r75:
Structure of the periplasmic binding protein AfuA from Actinobacillus pleuropneumoniae (exogenous sedoheptulose-7-phosphate bound)
Method: X-RAY DIFFRACTION / Resolution: 1.278 Å

Chemicals

ChemComp-GOL:
GLYCEROL

ChemComp-PEG:
DI(HYDROXYETHYL)ETHER

ChemComp-TRS:
2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL / pH buffer*YM

ChemComp-CL:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-G6P:
6-O-phosphono-alpha-D-glucopyranose

ChemComp-M6P:
6-O-phosphono-alpha-D-mannopyranose

ChemComp-F6P:
6-O-phosphono-beta-D-fructofuranose

ChemComp-MG:
Unknown entry

ChemComp-S7P:
1-C-(hydroxymethyl)-6-O-phosphono-beta-D-altrofuranose

ChemComp-PGE:
TRIETHYLENE GLYCOL

Source
  • actinobacillus pleuropneumoniae (bacteria)
KeywordsTRANSPORT PROTEIN / ABC transporter / sugar transporter / glucose-6-phosphate / fructose-6-phosphate / sedoheptulose-7-phosphate

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