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TitleA Cas9-guide RNA complex preorganized for target DNA recognition.
Journal, issue, pagesScience, Vol. 348, Page 1477-1481, Year 2015
Publish dateMay 14, 2015 (structure data deposition date)
AuthorsJiang, F. / Zhou, K. / Ma, L. / Gressel, S. / Doudna, J.A.
External linksScience / PubMed:26113724
MethodsX-ray diffraction
Resolution2.9 - 3.1 Å
Structure data

PDB-4zt0:
Crystal structure of catalytically-active Streptococcus pyogenes Cas9 in complex with single-guide RNA at 2.9 Angstrom resolution
Method: X-RAY DIFFRACTION / Resolution: 2.9 Å

PDB-4zt9:
Nuclease-inactive Streptococcus pyogenes Cas9 (D10A/H840A, dCas9) in complex with single-guide RNA at 3.1 Angstrom resolution
Method: X-RAY DIFFRACTION / Resolution: 3.1 Å

Source
  • streptococcus pyogenes (bacteria)
KeywordsHYDROLASE/RNA / CRISPR-Cas9 / bacteria adaptive immunity / DNA endonuclease / HYDROLASE-RNA complex / genome editing and regulation

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