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-Structure paper
タイトル | De novo protein structure determination from near-atomic-resolution cryo-EM maps. |
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ジャーナル・号・ページ | Nat Methods, Vol. 12, Issue 4, Page 335-338, Year 2015 |
掲載日 | 2015年2月23日 |
![]() | Ray Yu-Ruei Wang / Mikhail Kudryashev / Xueming Li / Edward H Egelman / Marek Basler / Yifan Cheng / David Baker / Frank DiMaio / ![]() ![]() |
PubMed 要旨 | We present a de novo model-building approach that combines predicted backbone conformations with side-chain fit to density to accurately assign sequence into density maps. This method yielded ...We present a de novo model-building approach that combines predicted backbone conformations with side-chain fit to density to accurately assign sequence into density maps. This method yielded accurate models for six of nine experimental maps at 3.3- to 4.8-Å resolution and produced a nearly complete model for an unsolved map containing a 660-residue heterodimeric protein. This method should enable rapid and reliable protein structure determination from near-atomic-resolution cryo-electron microscopy (cryo-EM) maps. |
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手法 | EM (単粒子) |
解像度 | 4.8 Å |
構造データ | ![]() EMDB-6219: |
由来 |
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