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TitleArchitecture of the RNA polymerase II-Mediator core initiation complex.
Journal, issue, pagesNature, Vol. 518, Issue 7539, Page 376-380, Year 2015
Publish dateFeb 19, 2015
AuthorsC Plaschka / L Larivière / L Wenzeck / M Seizl / M Hemann / D Tegunov / E V Petrotchenko / C H Borchers / W Baumeister / F Herzog / E Villa / P Cramer /
PubMed AbstractThe conserved co-activator complex Mediator enables regulated transcription initiation by RNA polymerase (Pol) II. Here we reconstitute an active 15-subunit core Mediator (cMed) comprising all ...The conserved co-activator complex Mediator enables regulated transcription initiation by RNA polymerase (Pol) II. Here we reconstitute an active 15-subunit core Mediator (cMed) comprising all essential Mediator subunits from Saccharomyces cerevisiae. The cryo-electron microscopic structure of cMed bound to a core initiation complex was determined at 9.7 Å resolution. cMed binds Pol II around the Rpb4-Rpb7 stalk near the carboxy-terminal domain (CTD). The Mediator head module binds the Pol II dock and the TFIIB ribbon and stabilizes the initiation complex. The Mediator middle module extends to the Pol II foot with a 'plank' that may influence polymerase conformation. The Mediator subunit Med14 forms a 'beam' between the head and middle modules and connects to the tail module that is predicted to bind transcription activators located on upstream DNA. The Mediator 'arm' and 'hook' domains contribute to a 'cradle' that may position the CTD and TFIIH kinase to stimulate Pol II phosphorylation.
External linksNature / PubMed:25652824
MethodsEM (single particle)
Resolution6.6 - 9.7 Å
Structure data

2784

4v1m

EMDB-2784, PDB-4v1m:
Architecture of the RNA polymerase II-Mediator core transcription initiation complex
Method: EM (single particle) / Resolution: 6.6 Å

2785

4v1n

EMDB-2785, PDB-4v1n:
Architecture of the RNA polymerase II-Mediator core transcription initiation complex
Method: EM (single particle) / Resolution: 7.8 Å

2786

4v1o

EMDB-2786, PDB-4v1o:
Architecture of the RNA polymerase II-Mediator core transcription initiation complex
Method: EM (single particle) / Resolution: 9.7 Å

Chemicals

ChemComp-ZN:
Unknown entry

ChemComp-MG:
Unknown entry

Source
  • saccharomyces cerevisiae (brewer's yeast)
  • synthetic construct (others)
  • saccharomyces mikatae (yeast)
KeywordsTRANSCRIPTION / TRANSCRIPTION INITIATION / RNA POLYMERASE II / GENERAL TRANSCRIPTION FACTORS

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