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Structure paper

TitleA Novel Polyamine Allosteric Site of SpeG from Vibrio cholerae Is Revealed by Its Dodecameric Structure.
Journal, issue, pagesJ. Mol. Biol., Vol. 427, Page 1316-1334, Year 2015
Publish dateMar 8, 2013 (structure data deposition date)
AuthorsFilippova, E.V. / Kuhn, M.L. / Osipiuk, J. / Kiryukhina, O. / Joachimiak, A. / Ballicora, M.A. / Anderson, W.F.
External linksJ. Mol. Biol. / PubMed:25623305
MethodsX-ray diffraction
Resolution1.848 - 2.83 Å
Structure data

PDB-4jjx:
Dodecameric structure of spermidine N-acetyltransferase SpeG from Vibrio cholerae O1 biovar eltor
Method: X-RAY DIFFRACTION / Resolution: 2.83 Å

PDB-4mhd:
Crystal structure of spermidine N-acetyltransferase from Vibrio cholerae in complex with spermidine
Method: X-RAY DIFFRACTION / Resolution: 2.32 Å

PDB-4mi4:
Crystal structure of spermidine N-acetyltransferase from Vibrio cholerae in complex with spermine
Method: X-RAY DIFFRACTION / Resolution: 1.848 Å

PDB-4ncz:
Spermidine N-acetyltransferase from Vibrio cholerae in complex with 2-[n-cyclohexylamino]ethane sulfonate.
Method: X-RAY DIFFRACTION / Resolution: 1.89 Å

PDB-4r57:
Crystal structure of spermidine N-acetyltransferase from Vibrio cholerae in complex with acetyl-CoA
Method: X-RAY DIFFRACTION / Resolution: 2.079 Å

PDB-4r87:
Crystal structure of spermidine N-acetyltransferase from Vibrio cholerae in complex with CoA and spermine
Method: X-RAY DIFFRACTION / Resolution: 2.61 Å

Chemicals

ChemComp-HOH:
WATER

ChemComp-SPD:
SPERMIDINE

ChemComp-SPM:
SPERMINE

ChemComp-NHE:
2-[N-CYCLOHEXYLAMINO]ETHANE SULFONIC ACID / pH buffer*YM

ChemComp-SO4:
SULFATE ION

ChemComp-CA:
Unknown entry

ChemComp-NA:
Unknown entry

ChemComp-ACO:
ACETYL COENZYME *A

ChemComp-PG4:
TETRAETHYLENE GLYCOL / precipitant*YM

ChemComp-PEG:
DI(HYDROXYETHYL)ETHER

ChemComp-COA:
COENZYME A

Source
  • vibrio cholerae o1 biovar el tor (bacteria)
  • vibrio cholerae (bacteria)
  • vibrio cholerae o1 (bacteria)
KeywordsTRANSFERASE / Structural Genomics / NIAID / National Institute of Allergy and Infectious Diseases / Center for Structural Genomics of Infectious Diseases / CSGID / spermidine / N-acetyltransferase / spermidine N1-acetyltransferase / IDP01616 / 2-[n-cyclohexylamino]ethane sulfonate / CHES / acetyl coenzyme A / coenzyme A / spermine

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