EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Molecular basis of histone tail recognition by human TIP5 PHD finger and bromodomain of the chromatin remodeling complex NoRC.
ジャーナル・号・ページ	Structure, Vol. 23, Issue 1, Page 80-92, Year 2015
掲載日	2015年1月6日
著者	Cynthia Tallant / Erica Valentini / Oleg Fedorov / Lois Overvoorde / Fleur M Ferguson / Panagis Filippakopoulos / Dmitri I Svergun / Stefan Knapp / Alessio Ciulli /
PubMed 要旨	Binding of the chromatin remodeling complex NoRC to RNA complementary to the rDNA promoter mediates transcriptional repression. TIP5, the largest subunit of NoRC, is involved in recruitment to rDNA ...Binding of the chromatin remodeling complex NoRC to RNA complementary to the rDNA promoter mediates transcriptional repression. TIP5, the largest subunit of NoRC, is involved in recruitment to rDNA by interactions with promoter-bound TTF-I, pRNA, and acetylation of H4K16. TIP5 domains that recognize posttranslational modifications on histones are essential for recruitment of NoRC to chromatin, but how these reader modules recognize site-specific histone tails has remained elusive. Here, we report crystal structures of PHD zinc finger and bromodomains from human TIP5 and BAZ2B in free form and bound to H3 and/or H4 histones. PHD finger functions as an independent structural module in recognizing unmodified H3 histone tails, and the bromodomain prefers H3 and H4 acetylation marks followed by a key basic residue, KacXXR. Further low-resolution analyses of PHD-bromodomain modules provide molecular insights into their trans histone tail recognition, required for nucleosome recruitment and transcriptional repression of the NoRC complex.
リンク	Structure / PubMed:25533489 / PubMed Central
手法	SAS (X-ray synchrotron) / X線回折
解像度	1.6 - 1.99 Å
構造データ	SASDA46: TIP5 in Tris (Bromodomain adjacent to zinc finger domain protein 2A, TIP5) 手法: SAXS/SANS SASDA56: BAZ2B in Tris (Bromodomain adjacent to zinc finger domain protein 2B, C-terminal, BAZ2B) 手法: SAXS/SANS SASDA66: BAZ2B-18mer complex in Tris (Bromodomain adjacent to zinc finger domain protein 2B, C-terminal, BAZ2B + H3Kac9Kac14, peptide 18mer) 手法: SAXS/SANS PDB-4lz2: Crystal structure of the bromodomain of human BAZ2A 手法: X-RAY DIFFRACTION / 解像度: 1.76 Å PDB-4q6f: Crystal structure of human BAZ2A PHD zinc finger in complex with unmodified H3K4 histone peptide 手法: X-RAY DIFFRACTION / 解像度: 1.91 Å PDB-4qbm: Crystal structure of human BAZ2A bromodomain in complex with a diacetylated histone 4 peptide (H4K16acK20ac) 手法: X-RAY DIFFRACTION / 解像度: 1.65 Å PDB-4qc1: Crystal structure of human BAZ2B bromodomain in complex with an acetylated histone 3 peptide (H3K14ac) 手法: X-RAY DIFFRACTION / 解像度: 1.99 Å PDB-4qc3: Crystal structure of human BAZ2B bromodomain in complex with a diacetylated histone 4 peptide (H4K8acK12ac) 手法: X-RAY DIFFRACTION / 解像度: 1.6 Å PDB-4qf2: Crystal structure of human BAZ2A PHD zinc finger in the free form 手法: X-RAY DIFFRACTION / 解像度: 1.7 Å PDB-4qf3: Crystal structure of human BAZ2B PHD zinc finger in the free form 手法: X-RAY DIFFRACTION / 解像度: 1.6 Å
化合物	ChemComp-EDO: 1,2-ETHANEDIOL / エチレングリコ-ル ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-HOH: WATER ChemComp-ZN: Unknown entry ChemComp-SO4: SULFATE ION / 硫酸ジアニオン ChemComp-PO4: PHOSPHATE ION / ホスファ-ト ChemComp-GOL: GLYCEROL / グリセロ-ル
由来	homo sapiens (ヒト)
キーワード	SIGNALING PROTEIN / Structural Genomics Consortium / SGC / TRANSCRIPTION / Bromodomain adjacent to zinc finger domain protein 2A / Transcription termination factor I-interacting protein 5 / TTF-I-interacting protein 5 / Tip5 / Bromodomain / Bromodomain adjacent to zinc finger domain protein 2B / hWALp4 / KIAA1476 / transcriptional regulation / transcriptional regulation interacting with ISWI