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- SASDA66: BAZ2B-18mer complex in Tris (Bromodomain adjacent to zinc finger ... -
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Open data
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Basic information
Entry | ![]() |
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![]() | BAZ2B-18mer complex in Tris
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Biological species | ![]() |
![]() | ![]() Title: Molecular basis of histone tail recognition by human TIP5 PHD finger and bromodomain of the chromatin remodeling complex NoRC. Authors: Cynthia Tallant / Erica Valentini / Oleg Fedorov / Lois Overvoorde / Fleur M Ferguson / Panagis Filippakopoulos / Dmitri I Svergun / Stefan Knapp / Alessio Ciulli / ![]() ![]() Abstract: Binding of the chromatin remodeling complex NoRC to RNA complementary to the rDNA promoter mediates transcriptional repression. TIP5, the largest subunit of NoRC, is involved in recruitment to rDNA ...Binding of the chromatin remodeling complex NoRC to RNA complementary to the rDNA promoter mediates transcriptional repression. TIP5, the largest subunit of NoRC, is involved in recruitment to rDNA by interactions with promoter-bound TTF-I, pRNA, and acetylation of H4K16. TIP5 domains that recognize posttranslational modifications on histones are essential for recruitment of NoRC to chromatin, but how these reader modules recognize site-specific histone tails has remained elusive. Here, we report crystal structures of PHD zinc finger and bromodomains from human TIP5 and BAZ2B in free form and bound to H3 and/or H4 histones. PHD finger functions as an independent structural module in recognizing unmodified H3 histone tails, and the bromodomain prefers H3 and H4 acetylation marks followed by a key basic residue, KacXXR. Further low-resolution analyses of PHD-bromodomain modules provide molecular insights into their trans histone tail recognition, required for nucleosome recruitment and transcriptional repression of the NoRC complex. |
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Structure visualization
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Downloads & links
-Models
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Sample
![]() | Name: BAZ2B-18mer complex in Tris / Specimen concentration: 2.75 mg/ml / Entity id: 115 / 117 |
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Buffer | Name: Tris / Concentration: 20.00 mM / pH: 8 Composition: NaCl 500.000 mM, DTT 2.000 mM, ZnCl2 10.000 microM |
Entity #115 | Name: BAZ2B / Type: protein Description: Bromodomain adjacent to zinc finger domain protein 2B, C-terminal Formula weight: 27.67 / Num. of mol.: 1 / Source: Homo sapiens Sequence: AMASIMKVYC QICRKGDNEE LLLLCDGCDK GCHTYCHRPK ITTIPDGDWF CPACIAKASG QTLKIKKLHV KGKKTNESKK GKKVTLTGDT EDEDSASTSS SLKRGNKDLK KRKMEENTSI NLSKQESFTS VKKPKRDDSK DLALCSMILT EMETHEDAWP FLLPVNLKLV ...Sequence: AMASIMKVYC QICRKGDNEE LLLLCDGCDK GCHTYCHRPK ITTIPDGDWF CPACIAKASG QTLKIKKLHV KGKKTNESKK GKKVTLTGDT EDEDSASTSS SLKRGNKDLK KRKMEENTSI NLSKQESFTS VKKPKRDDSK DLALCSMILT EMETHEDAWP FLLPVNLKLV PGYKKVIKKP MDFSTIREKL SSGQYPNLET FALDVRLVFD NCETFNEDDS DIGRAGHNMR KYFEKKWTDT FKVS |
Entity #117 | Name: peptide 18mer / Type: protein / Description: H3Kac9Kac14 / Formula weight: 4.7 / Num. of mol.: 1 / Source: Homo sapiens Sequence: ARTKQTARKS TGGKAPRK |
-Experimental information
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Detector | Name: Pilatus 1M-W / Pixsize x: 0.172 mm | ||||||||||||||||||||||||||||||
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