Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Detailed structural and biochemical characterization of the nexin-dynein regulatory complex.
Journal, issue, pages	Mol Biol Cell, Vol. 26, Issue 2, Page 294-304, Year 2015
Publish date	Jan 15, 2015
Authors	Toshiyuki Oda / Haruaki Yanagisawa / Masahide Kikkawa /
PubMed Abstract	The nexin-dynein regulatory complex (N-DRC) forms a cross-bridge between the outer doublet microtubules of the axoneme and regulates dynein motor activity in cilia/flagella. Although the molecular ...The nexin-dynein regulatory complex (N-DRC) forms a cross-bridge between the outer doublet microtubules of the axoneme and regulates dynein motor activity in cilia/flagella. Although the molecular composition and the three-dimensional structure of N-DRC have been studied using mutant strains lacking N-DRC subunits, more accurate approaches are necessary to characterize the structure and function of N-DRC. In this study, we precisely localized DRC1, DRC2, and DRC4 using cryo-electron tomography and structural labeling. All three N-DRC subunits had elongated conformations and spanned the length of N-DRC. Furthermore, we purified N-DRC and characterized its microtubule-binding properties. Purified N-DRC bound to the microtubule and partially inhibited microtubule sliding driven by the outer dynein arms (ODAs). Of interest, microtubule sliding was observed even in the presence of fourfold molar excess of N-DRC relative to ODA. These results provide insights into the role of N-DRC in generating the beating motions of cilia/flagella.
External links	Mol Biol Cell / PubMed:25411337 / PubMed Central
Methods	EM (subtomogram averaging)
Resolution	43.0 - 49.0 Å
Structure data	EMDB-6154: Detailed Structural and biochemical characterization of the nexin-dynein regulatory complex Method: EM (subtomogram averaging) / Resolution: 45.0 Å EMDB-6155: Detailed Structural and biochemical characterization of the nexin-dynein regulatory complex Method: EM (subtomogram averaging) / Resolution: 49.0 Å EMDB-6156: Detailed Structural and biochemical characterization of the nexin-dynein regulatory complex Method: EM (subtomogram averaging) / Resolution: 43.0 Å EMDB-6157: Detailed Structural and biochemical characterization of the nexin-dynein regulatory complex Method: EM (subtomogram averaging) / Resolution: 43.0 Å EMDB-6158: Detailed Structural and biochemical characterization of the nexin-dynein regulatory complex Method: EM (subtomogram averaging) / Resolution: 43.0 Å EMDB-6159: Detailed Structural and biochemical characterization of the nexin-dynein regulatory complex Method: EM (subtomogram averaging) / Resolution: 45.0 Å EMDB-6160: Detailed Structural and biochemical characterization of the nexin-dynein regulatory complex Method: EM (subtomogram averaging) / Resolution: 44.0 Å EMDB-6161: Detailed Structural and biochemical characterization of the nexin-dynein regulatory complex Method: EM (subtomogram averaging) / Resolution: 45.0 Å EMDB-6162: Detailed Structural and biochemical characterization of the nexin-dynein regulatory complex Method: EM (subtomogram averaging) / Resolution: 46.0 Å EMDB-6163: Detailed Structural and biochemical characterization of the nexin-dynein regulatory complex Method: EM (subtomogram averaging) / Resolution: 43.0 Å EMDB-6164: Detailed Structural and biochemical characterization of the nexin-dynein regulatory complex Method: EM (subtomogram averaging) / Resolution: 45.0 Å
Source	Chlamydomonas reinhardtii (plant)