EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Detailed structural and biochemical characterization of the nexin-dynein regulatory complex.
ジャーナル・号・ページ	Mol Biol Cell, Vol. 26, Issue 2, Page 294-304, Year 2015
掲載日	2015年1月15日
著者	Toshiyuki Oda / Haruaki Yanagisawa / Masahide Kikkawa /
PubMed 要旨	The nexin-dynein regulatory complex (N-DRC) forms a cross-bridge between the outer doublet microtubules of the axoneme and regulates dynein motor activity in cilia/flagella. Although the molecular ...The nexin-dynein regulatory complex (N-DRC) forms a cross-bridge between the outer doublet microtubules of the axoneme and regulates dynein motor activity in cilia/flagella. Although the molecular composition and the three-dimensional structure of N-DRC have been studied using mutant strains lacking N-DRC subunits, more accurate approaches are necessary to characterize the structure and function of N-DRC. In this study, we precisely localized DRC1, DRC2, and DRC4 using cryo-electron tomography and structural labeling. All three N-DRC subunits had elongated conformations and spanned the length of N-DRC. Furthermore, we purified N-DRC and characterized its microtubule-binding properties. Purified N-DRC bound to the microtubule and partially inhibited microtubule sliding driven by the outer dynein arms (ODAs). Of interest, microtubule sliding was observed even in the presence of fourfold molar excess of N-DRC relative to ODA. These results provide insights into the role of N-DRC in generating the beating motions of cilia/flagella.
リンク	Mol Biol Cell / PubMed:25411337 / PubMed Central
手法	EM (サブトモグラム平均)
解像度	43.0 - 49.0 Å
構造データ	EMDB-6154: Detailed Structural and biochemical characterization of the nexin-dynein regulatory complex 手法: EM (サブトモグラム平均) / 解像度: 45.0 Å EMDB-6155: Detailed Structural and biochemical characterization of the nexin-dynein regulatory complex 手法: EM (サブトモグラム平均) / 解像度: 49.0 Å EMDB-6156: Detailed Structural and biochemical characterization of the nexin-dynein regulatory complex 手法: EM (サブトモグラム平均) / 解像度: 43.0 Å EMDB-6157: Detailed Structural and biochemical characterization of the nexin-dynein regulatory complex 手法: EM (サブトモグラム平均) / 解像度: 43.0 Å EMDB-6158: Detailed Structural and biochemical characterization of the nexin-dynein regulatory complex 手法: EM (サブトモグラム平均) / 解像度: 43.0 Å EMDB-6159: Detailed Structural and biochemical characterization of the nexin-dynein regulatory complex 手法: EM (サブトモグラム平均) / 解像度: 45.0 Å EMDB-6160: Detailed Structural and biochemical characterization of the nexin-dynein regulatory complex 手法: EM (サブトモグラム平均) / 解像度: 44.0 Å EMDB-6161: Detailed Structural and biochemical characterization of the nexin-dynein regulatory complex 手法: EM (サブトモグラム平均) / 解像度: 45.0 Å EMDB-6162: Detailed Structural and biochemical characterization of the nexin-dynein regulatory complex 手法: EM (サブトモグラム平均) / 解像度: 46.0 Å EMDB-6163: Detailed Structural and biochemical characterization of the nexin-dynein regulatory complex 手法: EM (サブトモグラム平均) / 解像度: 43.0 Å EMDB-6164: Detailed Structural and biochemical characterization of the nexin-dynein regulatory complex 手法: EM (サブトモグラム平均) / 解像度: 45.0 Å
由来	Chlamydomonas reinhardtii (クラミドモナス)