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Structure paper

TitleComputational design of water-soluble alpha-helical barrels.
Journal, issue, pagesScience, Vol. 346, Page 485-488, Year 2014
Publish dateMay 23, 2014 (structure data deposition date)
AuthorsThomson, A.R. / Wood, C.W. / Burton, A.J. / Bartlett, G.J. / Sessions, R.B. / Brady, R.L. / Woolfson, D.N.
External linksScience / PubMed:25342807
MethodsX-ray diffraction
Resolution1.75 - 2.2 Å
Structure data

PDB-4pn8:
A de novo designed pentameric coiled coil CC-Pent.
Method: X-RAY DIFFRACTION / Resolution: 2 Å

PDB-4pn9:
A de novo designed hexameric coiled coil CC-Hex2
Method: X-RAY DIFFRACTION / Resolution: 2.2 Å

PDB-4pna:
A de novo designed heptameric coiled coil CC-Hept
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

PDB-4pnb:
A de novo designed hexameric coiled coil CC-Hex3.
Method: X-RAY DIFFRACTION / Resolution: 2.052 Å

PDB-4pnd:
A de novo designed pentameric coiled coil CC-Pent_Variant
Method: X-RAY DIFFRACTION / Resolution: 1.75 Å

Chemicals

ChemComp-HOH:
WATER

ChemComp-EPE:
4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID / pH buffer*YM

ChemComp-GOL:
GLYCEROL

Source
  • synthetic construct (others)
KeywordsDE NOVO PROTEIN / alpha-helical barrel / coiled coil / protein design / Artificially designed - often synthetic / peptide .

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