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-Structure paper
タイトル | Structure of catalase determined by MicroED. |
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ジャーナル・号・ページ | Elife, Vol. 3, Page e03600, Year 2014 |
掲載日 | 2014年10月10日 |
![]() | Brent L Nannenga / Dan Shi / Johan Hattne / Francis E Reyes / Tamir Gonen / ![]() |
PubMed 要旨 | MicroED is a recently developed method that uses electron diffraction for structure determination from very small three-dimensional crystals of biological material. Previously we used a series of ...MicroED is a recently developed method that uses electron diffraction for structure determination from very small three-dimensional crystals of biological material. Previously we used a series of still diffraction patterns to determine the structure of lysozyme at 2.9 Å resolution with MicroED (Shi et al., 2013). Here we present the structure of bovine liver catalase determined from a single crystal at 3.2 Å resolution by MicroED. The data were collected by continuous rotation of the sample under constant exposure and were processed and refined using standard programs for X-ray crystallography. The ability of MicroED to determine the structure of bovine liver catalase, a protein that has long resisted atomic analysis by traditional electron crystallography, demonstrates the potential of this method for structure determination. |
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手法 | EM (電子線結晶学) |
解像度 | 3.2 Å |
構造データ | |
化合物 | ![]() ChemComp-HEM: ![]() ChemComp-NDP: |
由来 |
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![]() | OXIDOREDUCTASE |