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Structure paper

TitleBiochemical and structural characterization of a thermostable beta-glucosidase from Halothermothrix orenii for galacto-oligosaccharide synthesis.
Journal, issue, pagesAppl. Microbiol. Biotechnol., Vol. 99, Page 1731-1744, Year 2015
Publish dateMar 11, 2014 (structure data deposition date)
AuthorsHassan, N. / Nguyen, T.H. / Intanon, M. / Kori, L.D. / Patel, B.K. / Haltrich, D. / Divne, C. / Tan, T.C.
External linksAppl. Microbiol. Biotechnol. / PubMed:25173693
MethodsX-ray diffraction
Resolution1.8 - 2 Å
Structure data

PDB-4ptv:
Halothermothrix orenii beta-glucosidase A, thiocellobiose complex
Method: X-RAY DIFFRACTION / Resolution: 1.85 Å

PDB-4ptw:
Halothermothrix orenii beta-glucosidase A, 2-deoxy-2-fluoro-glucose complex
Method: X-RAY DIFFRACTION / Resolution: 2 Å

PDB-4ptx:
Halothermothrix orenii beta-glucosidase A, glucose complex
Method: X-RAY DIFFRACTION / Resolution: 1.8 Å

Chemicals

ChemComp-CS:
Unknown entry

ChemComp-PE4:
2-{2-[2-(2-{2-[2-(2-ETHOXY-ETHOXY)-ETHOXY]-ETHOXY}-ETHOXY)-ETHOXY]-ETHOXY}-ETHANOL / precipitant*YM

ChemComp-HOH:
WATER

ChemComp-G2F:
2-deoxy-2-fluoro-alpha-D-glucopyranose

ChemComp-PG4:
TETRAETHYLENE GLYCOL / precipitant*YM

ChemComp-BGC:
beta-D-glucopyranose

ChemComp-CAC:
CACODYLATE ION

Source
  • halothermothrix orenii (bacteria)
KeywordsHYDROLASE / (beta/alpha)8 fold / TIM barrel / glycoside hydrolase / beta-glucosidase

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