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Structure paper

TitleStructural Basis of Pam-Dependent Target DNA Recognition by the Cas9 Endonuclease
Journal, issue, pagesNature, Vol. 513, Page 569-, Year 2014
Publish dateMay 25, 2014 (structure data deposition date)
AuthorsAnders, C. / Niewoehner, O. / Duerst, A. / Jinek, M.
External linksNature / PubMed:25079318
MethodsX-ray diffraction
Resolution2.371 - 2.593 Å
Structure data

PDB-4un3:
Crystal structure of Cas9 bound to PAM-containing DNA target
Method: X-RAY DIFFRACTION / Resolution: 2.593 Å

PDB-4un4:
Crystal structure of Cas9 bound to PAM-containing DNA target with mismatches at positions 1-2
Method: X-RAY DIFFRACTION / Resolution: 2.371 Å

PDB-4un5:
Crystal structure of Cas9 bound to PAM-containing DNA target containing mismatches at positions 1-3
Method: X-RAY DIFFRACTION / Resolution: 2.4 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-HOH:
WATER

Source
  • streptococcus pyogenes (bacteria)
  • synthetic construct (others)
KeywordsHYDROLASE/DNA/RNA / HYDROLASE-DNA-RNA COMPLEX / CRISPR / GENOME EDITING / RNP / PROTEIN-RNA COMPLEX

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