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Structure paper

TitleThe structure of a transient complex of a nonribosomal Peptide synthetase and a cytochrome p450 monooxygenase.
Journal, issue, pagesAngew. Chem. Int. Ed. Engl., Vol. 53, Page 8518-8522, Year 2014
Publish dateMar 21, 2014 (structure data deposition date)
AuthorsHaslinger, K. / Brieke, C. / Uhlmann, S. / Sieverling, L. / Sussmuth, R.D. / Cryle, M.J.
External linksAngew. Chem. Int. Ed. Engl. / PubMed:25044735
MethodsX-ray diffraction
Resolution2.7 - 3 Å
Structure data

PDB-4pwv:
Structure of P450sky (CYP163B3), a cytochrome P450 from skyllamycin biosynthesis in complex with a peptidyl carrier protein domain
Method: X-RAY DIFFRACTION / Resolution: 3 Å

PDB-4pxh:
Structure of P450sky (CYP163B3), a cytochrome P450 from skyllamycin biosynthesis in complex with a peptidyl carrier protein domain
Method: X-RAY DIFFRACTION / Resolution: 2.7 Å

Chemicals

ChemComp-HEM:
PROTOPORPHYRIN IX CONTAINING FE

ChemComp-KH4:
S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] 1H-imidazole-4-carbothioate

ChemComp-HOH:
WATER

Source
  • streptomyces sp. acta 2897 (bacteria)
KeywordsOXIDOREDUCTASE/PROTEIN BINDING / Cytochrome P450 fold / beta-aminoacyl carrier protein hydroxylase / peptidyl carrier protein domains / skyllamycin NRPS / OXIDOREDUCTASE-PROTEIN BINDING complex

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