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Structure paper

TitleStructural basis for the binding and incorporation of nucleotide analogs with L-stereochemistry by human DNA polymerase lambda.
Journal, issue, pagesProc. Natl. Acad. Sci. USA, Vol. 111, Page E3033-E3042, Year 2014
Publish dateApr 12, 2013 (structure data deposition date)
AuthorsVyas, R. / Zahurancik, W.J. / Suo, Z.
External linksProc. Natl. Acad. Sci. USA / PubMed:25015085
MethodsX-ray diffraction
Resolution2.1 - 2.25 Å
Structure data

PDB-4k4g:
Ternary crystal structures of human DNA POLYMERASE LAMBDA IN COMPLEX WITH DNA AND L-DCTP.
Method: X-RAY DIFFRACTION / Resolution: 2.15 Å

PDB-4k4h:
Ternary crystal structures of a human DNA POLYMERASE LAMBDA IN COMPLEX WITH DNA AND (-)3TC-TP.
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

PDB-4k4i:
Ternary crystal structures of a human DNA POLYMERASE LAMBDA IN COMPLEX WITH DNA AND (-)FTC-TP.
Method: X-RAY DIFFRACTION / Resolution: 2.25 Å

Chemicals

ChemComp-1S0:
4-amino-1-{2-deoxy-5-O-[(R)-hydroxy{[(S)-hydroxy(phosphonooxy)phosphoryl]oxy}phosphoryl]-beta-L-erythro-pentofuranosyl}pyrimidin-2(1H)-one

ChemComp-CA:
Unknown entry

ChemComp-ACT:
ACETATE ION

ChemComp-CAC:
CACODYLATE ION

ChemComp-HOH:
WATER

ChemComp-1RZ:
Lamivudine Triphosphate

ChemComp-1RY:
[[(2R,5S)-5-(4-azanyl-5-fluoranyl-2-oxidanylidene-pyrimidin-1-yl)-1,3-oxathiolan-2-yl]methoxy-oxidanyl-phosphoryl] phosphono hydrogen phosphate

Source
  • homo sapiens (human)
  • synthetic construct (others)
KeywordsTRANSFERASE/DNA / DNA POLYMERASE / DNA REPAIR / PHOSPHORYL TRANSFER REACTION / TRANSFERASE-DNA complex

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