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TitleGeneration of a high-fidelity antibody against nerve growth factor using library scanning mutagenesis and validation with structures of the initial and optimized Fab-antigen complexes.
Journal, issue, pagesMAbs, Vol. 6, Page 1059-1068
Publish dateMar 27, 2012 (structure data deposition date)
AuthorsLa Porte, S.L. / Eigenbrot, C. / Ultsch, M. / Ho, W.H. / Foletti, D. / Forgie, A. / Lindquist, K.C. / Shelton, D.L. / Pons, J.
External linksPubMed:24830649
MethodsX-ray diffraction
Resolution2.48 - 2.5 Å
Structure data

PDB-4edw:
Nerve Growth Factor in Complex with Fab from humanized version of mouse mAb 911 (tanezumab)
Method: X-RAY DIFFRACTION / Resolution: 2.48 Å

PDB-4edx:
Nerve Growth Factor in Complex with Fab from mouse mAb 911
Method: X-RAY DIFFRACTION / Resolution: 2.5 Å

Chemicals

ChemComp-GOL:
GLYCEROL

ChemComp-HOH:
WATER

Source
  • homo sapiens (human)
  • mus musculus (house mouse)
KeywordsIMMUNE SYSTEM / cystine knot / immunoglobulin / growth/survival factor

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