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Structure paper

TitleArchitecture of a single membrane spanning cytochrome P450 suggests constraints that orient the catalytic domain relative to a bilayer.
Journal, issue, pagesProc. Natl. Acad. Sci. USA, Vol. 111, Page 3865-3870, Year 2014
Publish dateJul 29, 2013 (structure data deposition date)
AuthorsMonk, B.C. / Tomasiak, T.M. / Keniya, M.V. / Huschmann, F.U. / Tyndall, J.D. / O'Connell, J.D. / Cannon, R.D. / McDonald, J.G. / Rodriguez, A. / Finer-Moore, J.S. / Stroud, R.M.
External linksProc. Natl. Acad. Sci. USA / PubMed:24613931
MethodsX-ray diffraction
Resolution1.9 - 2.19 Å
Structure data

PDB-4lxj:
Saccharomyces cerevisiae lanosterol 14-alpha demethylase with lanosterol bound
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

PDB-5eqb:
Crystal structure of lanosterol 14-alpha demethylase with intact transmembrane domain bound to itraconazole
Method: X-RAY DIFFRACTION / Resolution: 2.19 Å

Chemicals

ChemComp-HEM:
PROTOPORPHYRIN IX CONTAINING FE

ChemComp-LAN:
LANOSTEROL

ChemComp-OXY:
OXYGEN MOLECULE

ChemComp-HOH:
WATER

ChemComp-1YN:
2-[(2R)-butan-2-yl]-4-{4-[4-(4-{[(2R,4S)-2-(2,4-dichlorophenyl)-2-(1H-1,2,4-triazol-1-ylmethyl)-1,3-dioxolan-4-yl]methoxy}phenyl)piperazin-1-yl]phenyl}-2,4-dihydro-3H-1,2,4-triazol-3-one

Source
  • saccharomyces cerevisiae (brewer's yeast)
  • saccharomyces cerevisiae (strain atcc 204508 / s288c) (yeast)
KeywordsOXIDOREDUCTASE / Cytochrome p450 / oxidoreductase/oxidoreductase inhibitor / Sterol Antifungal Membrane Cytochrome / Structural Genomics / PSI-2 / Protein Structure Initiative / Center for Structures of Membrane Proteins / CSMP / oxidoreductase-oxidoreductase inhibitor complex

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