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TitleFirst Structural Insights into alpha-L-Arabinofuranosidases from the Two GH62 Glycoside Hydrolase Subfamilies.
Journal, issue, pagesJ. Biol. Chem., Vol. 289, Page 5261-5273, Year 2014
Publish dateOct 4, 2013 (structure data deposition date)
AuthorsSiguier, B. / Haon, M. / Nahoum, V. / Marcellin, M. / Burlet-Schiltz, O. / Coutinho, P.M. / Henrissat, B. / Mourey, L. / O'Donohue, M.J. / Berrin, J.G. ...Siguier, B. / Haon, M. / Nahoum, V. / Marcellin, M. / Burlet-Schiltz, O. / Coutinho, P.M. / Henrissat, B. / Mourey, L. / O'Donohue, M.J. / Berrin, J.G. / Tranier, S. / Dumon, C.
External linksJ. Biol. Chem. / PubMed:24394409
MethodsX-ray diffraction
Resolution1 - 1.8 Å
Structure data

PDB-4n1i:
Crystal Structure of the alpha-L-arabinofuranosidase UmAbf62A from Ustilago maidys
Method: X-RAY DIFFRACTION / Resolution: 1 Å

PDB-4n2r:
Crystal Structure of the alpha-L-arabinofuranosidase UmAbf62A from Ustilago maydis in complex with L-arabinofuranose
Method: X-RAY DIFFRACTION / Resolution: 1.2 Å

PDB-4n2z:
Crystal Structure of the alpha-L-arabinofuranosidase PaAbf62A from Podospora anserina in complex with cellotriose
Method: X-RAY DIFFRACTION / Resolution: 1.8 Å

PDB-4n4b:
Crystal Structure of the alpha-L-arabinofuranosidase PaAbf62A from Podospora anserina
Method: X-RAY DIFFRACTION / Resolution: 1.44 Å

Chemicals

ChemComp-CA:
Unknown entry

ChemComp-GOL:
GLYCEROL

ChemComp-TRS:
2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL / pH buffer*YM

ChemComp-1PE:
PENTAETHYLENE GLYCOL / precipitant*YM

ChemComp-HOH:
WATER

ChemComp-FUB:
beta-L-arabinofuranose

ChemComp-AHR:
alpha-L-arabinofuranose

ChemComp-EPE:
4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID / pH buffer*YM

Source
  • ustilago maydis (corn smut)
  • podospora anserina (fungus)
KeywordsHYDROLASE / Beta-Propeller / hemicellulose binding

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