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TitleThe ABC-F protein EttA gates ribosome entry into the translation elongation cycle.
Journal, issue, pagesNat Struct Mol Biol, Vol. 21, Issue 2, Page 143-151, Year 2014
Publish dateJan 5, 2014
AuthorsGrégory Boël / Paul C Smith / Wei Ning / Michael T Englander / Bo Chen / Yaser Hashem / Anthony J Testa / Jeffrey J Fischer / Hans-Joachim Wieden / Joachim Frank / Ruben L Gonzalez / John F Hunt /
PubMed AbstractABC-F proteins have evaded functional characterization even though they compose one of the most widely distributed branches of the ATP-binding cassette (ABC) superfamily. Herein, we demonstrate that ...ABC-F proteins have evaded functional characterization even though they compose one of the most widely distributed branches of the ATP-binding cassette (ABC) superfamily. Herein, we demonstrate that YjjK, the most prevalent eubacterial ABC-F protein, gates ribosome entry into the translation elongation cycle through a nucleotide-dependent interaction sensitive to ATP/ADP ratio. Accordingly, we rename this protein energy-dependent translational throttle A (EttA). We determined the crystal structure of Escherichia coli EttA and used it to design mutants for biochemical studies including enzymological assays of the initial steps of protein synthesis. These studies suggest that EttA may regulate protein synthesis in energy-depleted cells, which have a low ATP/ADP ratio. Consistently with this inference, EttA-deleted cells exhibit a severe fitness defect in long-term stationary phase. These studies demonstrate that an ABC-F protein regulates protein synthesis via a new mechanism sensitive to cellular energy status.
External linksNat Struct Mol Biol / PubMed:24389466 / PubMed Central
MethodsX-ray diffraction
Resolution2.4 Å
Structure data

PDB-4fin:
Crystal Structure of EttA (formerly YjjK) - an E. coli ABC-type ATPase
Method: X-RAY DIFFRACTION / Resolution: 2.4 Å

Chemicals

ChemComp-SO4:
SULFATE ION / Sulfate

ChemComp-CIT:
CITRIC ACID / Citric acid

ChemComp-PGE:
TRIETHYLENE GLYCOL / Polyethylene glycol

ChemComp-GOL:
GLYCEROL / Glycerol

ChemComp-HOH:
WATER / Water

Source
  • escherichia coli (E. coli)
KeywordsATP-BINDING PROTEIN / ABC protein / ABCF / mechanoenzyme / Gating of ribosomal elongation / Ribosome / Cytosol/Ribosome / Northeast Structural Genomics Consortium / NESG / PSI-Biology / structural genomics

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