Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Visualization of distinct substrate-recruitment pathways in the yeast exosome by EM.
Journal, issue, pages	Nat Struct Mol Biol, Vol. 21, Issue 1, Page 95-9102, Year 2014
Publish date	Dec 15, 2013
Authors	Jun-Jie Liu / Matthew A Bratkowski / Xueqi Liu / Chu-Ya Niu / Ailong Ke / Hong-Wei Wang /
PubMed Abstract	The eukaryotic exosome is a multisubunit complex typically composed of a catalytically inactive core and the Rrp44 protein, which contains 3'-to-5' exo- and endo-RNase activities. RNA substrates have ...The eukaryotic exosome is a multisubunit complex typically composed of a catalytically inactive core and the Rrp44 protein, which contains 3'-to-5' exo- and endo-RNase activities. RNA substrates have been shown to be recruited through the core to reach Rrp44's exo-RNase (EXO) site. Using single-particle EM and biochemical analysis, we provide visual evidence that two distinct substrate-recruitment pathways exist. In the through-core route, channeling of the single-stranded substrates from the core to Rrp44 induces a characteristic conformational change in Rrp44. In the alternative direct-access route, this conformational change does not take place, and the RNA substrate is visualized to avoid the core and enter Rrp44's EXO site directly. Our results provide mechanistic explanations for several RNA processing scenarios by the eukaryotic exosome and indicate substrate-specific modes of degradation by this complex.
External links	Nat Struct Mol Biol / PubMed:24336220 / PubMed Central
Methods	EM (single particle)
Resolution	23.0 - 26.0 Å
Structure data	EMDB-2491: Recruitment Pathways in the Yeast Exosome by Electron Microscopy Method: EM (single particle) / Resolution: 25.0 Å EMDB-2492: Substrate Recruitment Pathways in the Yeast Exosome by Electron Microscopy Method: EM (single particle) / Resolution: 25.0 Å EMDB-2493: Substrate Recruitment Pathways in the Yeast Exosome by Electron Microscopy Method: EM (single particle) / Resolution: 25.0 Å EMDB-2494: Substrate Recruitment Pathways in the Yeast Exosome by Electron Microscopy Method: EM (single particle) / Resolution: 23.0 Å EMDB-2495: Substrate Recruitment Pathways in the Yeast Exosome by Electron Microscopy Method: EM (single particle) / Resolution: 23.0 Å EMDB-2496: Substrate Recruitment Pathways in the Yeast Exosome by Electron Microscopy Method: EM (single particle) / Resolution: 23.0 Å EMDB-2497: Substrate Recruitment Pathways in the Yeast Exosome by Electron Microscopy Method: EM (single particle) / Resolution: 23.0 Å EMDB-2498: Substrate Recruitment Pathways in the Yeast Exosome by Electron Microscopy Method: EM (single particle) / Resolution: 23.0 Å EMDB-2499: Substrate Recruitment Pathways in the Yeast Exosome by Electron Microscopy Method: EM (single particle) / Resolution: 26.0 Å EMDB-2500: Substrate Recruitment Pathways in the Yeast Exosome by Electron Microscopy Method: EM (single particle) / Resolution: 25.0 Å EMDB-2501: Substrate Recruitment Pathways in the Yeast Exosome by Electron Microscopy Method: EM (single particle) / Resolution: 24.0 Å EMDB-2502: Substrate Recruitment Pathways in the Yeast Exosome by Electron Microscopy Method: EM (single particle) / Resolution: 24.0 Å EMDB-2522: Substrate Recruitment Pathways in the Yeast Exosome by Electron Microscopy Method: EM (single particle) / Resolution: 23.0 Å
Source	Saccharomyces cerevisiae (brewer's yeast) synthetic construct (others) Escherichia coli (E. coli)