+検索条件
-Structure paper
タイトル | Structure of the dodecameric Yersinia enterocolitica secretin YscC and its trypsin-resistant core. |
---|---|
ジャーナル・号・ページ | Structure, Vol. 21, Issue 12, Page 2152-2161, Year 2013 |
掲載日 | 2013年12月3日 |
著者 | Julia Kowal / Mohamed Chami / Philippe Ringler / Shirley A Müller / Mikhail Kudryashev / Daniel Castaño-Díez / Marlise Amstutz / Guy R Cornelis / Henning Stahlberg / Andreas Engel / |
PubMed 要旨 | The type III secretion system machinery, also known as the injectisome, delivers bacterial effector proteins into eukaryotic cells during infection. The outer membrane YscC secretin is a major part ...The type III secretion system machinery, also known as the injectisome, delivers bacterial effector proteins into eukaryotic cells during infection. The outer membrane YscC secretin is a major part of Yersinia enterocolitica's injectisome and is among the first components to assemble, solely assisted by its pilotin, YscW. We have determined the three-dimensional structures of the native complex and its protease-resistant core to 12 Å resolution by cryo-electron microscopy (cryo-EM) and show that YscC forms a dodecameric complex. Cryo-EM of YscC reconstituted into proteoliposomes defines the secretin's membrane-spanning region. Native YscC consists of an outer membrane ring connected via a thin cylindrical wall to a conical, periplasmic region that exposes N-terminal petals connected by flexible linkers. These petals harbor the binding site of YscD, a component of the inner membrane ring. A change in their orientation adapts the length of the YscC secretin and facilitates its interaction with YscD. |
リンク | Structure / PubMed:24207124 |
手法 | EM (単粒子) |
解像度 | 15.0 Å |
構造データ | EMDB-5720: EMDB-5721: EMDB-5722: |
由来 |
|