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Structure paper

TitlePlug-and-Play Pairing Via Defined Divalent Streptavidins.
Journal, issue, pagesJ. Mol. Biol., Vol. 426, Page 199-, Year 2014
Publish dateJul 8, 2013 (structure data deposition date)
AuthorsFairhead, M. / Krndija, D. / Lowe, E.D. / Howarth, M.
External linksJ. Mol. Biol. / PubMed:24056174
MethodsX-ray diffraction
Resolution1.431 - 2.26 Å
Structure data

PDB-4bx5:
cis-divalent streptavidin
Method: X-RAY DIFFRACTION / Resolution: 1.431 Å

PDB-4bx6:
trans-divalent streptavidin
Method: X-RAY DIFFRACTION / Resolution: 1.595 Å

PDB-4bx7:
trans-divalent streptavidin bound to biotin-4-fluorescein
Method: X-RAY DIFFRACTION / Resolution: 2.26 Å

Chemicals

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-PG4:
TETRAETHYLENE GLYCOL / precipitant*YM

ChemComp-HOH:
WATER

ChemComp-MPD:
(4S)-2-METHYL-2,4-PENTANEDIOL / precipitant*YM

ChemComp-B4F:
biotin-4-fluorescein

Source
  • streptomyces avidinii (bacteria)
KeywordsBIOTIN-BINDING PROTEIN / BIOTIN / AVIDIN

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