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-Structure paper
タイトル | Structural mimicry in transcription regulation of human RNA polymerase II by the DNA helicase RECQL5. |
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ジャーナル・号・ページ | Nat Struct Mol Biol, Vol. 20, Issue 7, Page 892-899, Year 2013 |
掲載日 | 2013年6月9日 |
![]() | Susanne A Kassube / Martin Jinek / Jie Fang / Susan Tsutakawa / Eva Nogales / ![]() |
PubMed 要旨 | RECQL5 is a member of the highly conserved RecQ family of DNA helicases involved in DNA repair. RECQL5 interacts with RNA polymerase II (Pol II) and inhibits transcription of protein-encoding genes ...RECQL5 is a member of the highly conserved RecQ family of DNA helicases involved in DNA repair. RECQL5 interacts with RNA polymerase II (Pol II) and inhibits transcription of protein-encoding genes by an unknown mechanism. We show that RECQL5 contacts the Rpb1 jaw domain of Pol II at a site that overlaps with the binding site for the transcription elongation factor TFIIS. Our cryo-EM structure of elongating Pol II arrested in complex with RECQL5 shows that the RECQL5 helicase domain is positioned to sterically block elongation. The crystal structure of the RECQL5 KIX domain reveals similarities with TFIIS, and binding of RECQL5 to Pol II interferes with the ability of TFIIS to promote transcriptional read-through in vitro. Together, our findings reveal a dual mode of transcriptional repression by RECQL5 that includes structural mimicry of the Pol II-TFIIS interaction. |
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手法 | EM (単粒子) / X線回折 |
解像度 | 1.9 - 13.0 Å |
構造データ | ![]() EMDB-2367: ![]() PDB-4bk0: |
化合物 | ![]() ChemComp-PEG: ![]() ChemComp-HOH: |
由来 |
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![]() | TRANSCRIPTION / DNA HELICASE / TRANSCRIPTIONAL REPRESSION |