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-Structure paper
タイトル | Atomic structure of the 75 MDa extremophile Sulfolobus turreted icosahedral virus determined by CryoEM and X-ray crystallography. |
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ジャーナル・号・ページ | Proc Natl Acad Sci U S A, Vol. 110, Issue 14, Page 5504-5509, Year 2013 |
掲載日 | 2013年4月2日 |
著者 | David Veesler / Thiam-Seng Ng / Anoop K Sendamarai / Brian J Eilers / C Martin Lawrence / Shee-Mei Lok / Mark J Young / John E Johnson / Chi-yu Fu / |
PubMed 要旨 | Sulfolobus turreted icosahedral virus (STIV) was isolated in acidic hot springs where it infects the archeon Sulfolobus solfataricus. We determined the STIV structure using near-atomic resolution ...Sulfolobus turreted icosahedral virus (STIV) was isolated in acidic hot springs where it infects the archeon Sulfolobus solfataricus. We determined the STIV structure using near-atomic resolution electron microscopy and X-ray crystallography allowing tracing of structural polypeptide chains and visualization of transmembrane proteins embedded in the viral membrane. We propose that the vertex complexes orchestrate virion assembly by coordinating interactions of the membrane and various protein components involved. STIV shares the same coat subunit and penton base protein folds as some eukaryotic and bacterial viruses, suggesting that they derive from a common ancestor predating the divergence of the three kingdoms of life. One architectural motif (β-jelly roll fold) forms virtually the entire capsid (distributed in three different gene products), indicating that a single ancestral protein module may have been at the origin of its evolution. |
リンク | Proc Natl Acad Sci U S A / PubMed:23520050 / PubMed Central |
手法 | EM (単粒子) / X線回折 |
解像度 | 1.4 - 4.5 Å |
構造データ | EMDB-5584, PDB-3j31: PDB-4il7: |
化合物 | ChemComp-HOH: |
由来 |
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キーワード | VIRUS / Virus assembly / Evolution / Archaea / VIRAL PROTEIN / partial jelly roll fold / hypothetical protein / C381 and B345 / Viral Capsid |